ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa
|version 3 (curated until May 28, 2011)|
Toxoplasma gondii sequesters centromeres to a specific nuclear region throughout the cell cycle.
Brooks, C. F., Francia, M. E., Gissot, M., Croken, M. M., Kim, K., Striepen, B. (2011 Mar 1, Proc Natl Acad Sci U S A)
Members of the eukaryotic phylum Apicomplexa are the cause of important human diseases including malaria, toxoplasmosis, and cryptosporidiosis. These obligate intracellular parasites produce new invasive stages through a complex budding process. The budding cycle is remarkably flexible and can produce varied numbers of progeny to adapt to different host-cell niches. How this complex process is coordinated remains poorly understood. Using Toxoplasma gondii as a genetic model, we show that a key element to this coordination is the centrocone, a unique elaboration of the nuclear envelope that houses the mitotic spindle. Exploiting transgenic parasite lines expressing epitope-tagged centromeric H3 variant CenH3, we identify the centromeres of T. gondii chromosomes by hybridization of chromatin immunoprecipitations to genome-wide microarrays (ChIP-chip). We demonstrate that centromere attachment to the centrocone persists throughout the parasite cell cycle and that centromeres localize to a single apical region within the nucleus. Centromere sequestration provides a mechanism for the organization of the Toxoplasma nucleus and the maintenance of genome integrity.
TGME49_025410 (CenH3, CenpA) histone H3 variant, putativeExperimental localisation: nucleus during tachyzoite
TGME49_061240 (H3) histone H3Experimental localisation: nucleus during tachyzoite
TGME49_018260 (H3.3) histone H3.3 variantExperimental localisation: nucleus during tachyzoite