ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Functional dissection of the apicomplexan glideosome molecular architecture.

Frenal, K., Polonais, V., Marq, J. B., Stratmann, R., Limenitakis, J., Soldati-Favre, D. (2010 Oct 21, Cell Host Microbe)

The glideosome of apicomplexan parasites is an actin- and myosin-based machine located at the pellicle, between the plasma membrane (PM) and inner membrane complex (IMC), that powers parasite motility, migration, and host cell invasion and egress. It is composed of myosin A, its light chain MLC1, and two gliding-associated proteins, GAP50 and GAP45. We identify GAP40, a polytopic protein of the IMC, as an additional glideosome component and show that GAP45 is anchored to the PM and IMC via its N- and C-terminal extremities, respectively. While the C-terminal region of GAP45 recruits MLC1-MyoA to the IMC, the N-terminal acylation and coiled-coil domain preserve pellicle integrity during invasion. GAP45 is essential for gliding, invasion, and egress. The orthologous Plasmodium falciparum GAP45 can fulfill this dual function, as shown by transgenera complementation, whereas the coccidian GAP45 homolog (designated here as) GAP70 specifically recruits the glideosome to the apical cap of the parasite.

PubMed: 20951968, full text

Localisation information

TGME49_049850 (GAP40) hypothetical protein, conserved

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "The generation of transgenic parasites expressing a C-terminal tagged version of this protein identified a resident polytopic protein of the IMC with an apparent molecular weight of ~37 kDa (Figures 1B and 1C)."
  • Microscopy type: fixed light
  • Microscopy method: Ty tag
  • Strain: RH
  • Gene model mapping comments: blast from HM751080
  • Localisation record: inner membrane complex

TGME49_023940 (GAP45) hypothetical protein

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Both MLC1Ty and NtMLC1GFPTy partitioned with the IMC, colocalizing with GAP45, like the endogenous MLC1"
  • Microscopy type: fixed light
  • Microscopy method: antibody directly to protein
  • Strain: RH
  • Gene model mapping comments: inferred from another publication
  • Localisation record: IMC

TGME49_057680 (MLC1) myosin light chain TgMLC1

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Both MLC1Ty and NtMLC1GFPTy partitioned with the IMC, colocalizing with GAP45, like the endogenous MLC1"
  • Microscopy type: fixed light
  • Microscopy method: Ty tag
  • Strain: RH
  • Gene model mapping comments: inferred from another publication
  • Localisation record: IMC

TGME49_033030 (GAP70) myosin-A docking protein, putative

Experimental localisation: apical cap during tachyzoite, inner membrane complex during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "By IFA, TyGAP70 was concentrated at the apical cap but also distributed throughout the entire pellicle,"
  • Microscopy type: fixed light
  • Microscopy method: Ty tag
  • Strain: RH
  • Gene model mapping comments: blast from HM117968, gene model inconsistent?
  • Localisation record: concentrated apical cap and pellicle during tachyzoite