ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Novel roles for ATP-binding cassette G transporters in lipid redistribution in Toxoplasma.

Ehrenman, K., Sehgal, A., Lige, B., Stedman, T. T., Joiner, K. A., Coppens, I. (2010 Jun 1, Mol Microbiol)

Toxoplasma is a protozoan parasite proficiently adapted to thrive in a parasitophorous vacuole (PV) formed in the cytoplasm of a large variety of mammalian cells. As an actively dividing organism, the parasite must adjust the lipid composition of its membranes to preserve organelle vitality and expand the size of the PV membrane to accommodate growing progeny. We showed that Toxoplasma takes up host lipids and can expel major lipids in an ATP-dependent process. In order to provide detailed mechanistic insights into lipid trafficking phenomena relevant to Toxoplasma biology, we characterized six parasite ATP-binding cassette (ABC) G family transporters and investigated their potential contribution to lipid homeostatic processes. All these transporters are expressed in the parasite and five of them are upregulated upon exposure to sterols. Four ABCG are localized to secretory organelles and the plasma membrane, and promote cholesterol and phospholipid efflux, reflecting the importance in exportation of large amounts of lipids into the PV. Interestingly, one ABCG that is associated with vesicles in the PV and the plasma membrane acts as a cholesterol importer. This last finding expands our current view on the role of some ABCG transporters in eukaryotic sterol influx.

PubMed: 20487267, full text

Localisation information

TGME49_090580 (ABCG89) ABC transporter, putative

Experimental localisation: endoplasmic reticulum during intracellular tachyzoite, cytoplasmic vesicle during intracellular tachyzoite, peripheral during intracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "We observed a specific staining of the ER, internal vesicles and cell periphery for TgABCG89, TgABCG84, TgABCG87 and TgABCG96 in intracellular parasites."
  • Microscopy type: light, EM
  • Microscopy method: antibody
  • Strain: RH
  • Gene model mapping comments: blast from DQ099411, gene model inconsistent?
  • Localisation record: ER and internal vesicles and cell periphery during intracellular tachyzoite

TGME49_089350 (ABCG84) white protein, putative

Experimental localisation: endoplasmic reticulum during intracellular tachyzoite, cytoplasmic vesicle during intracellular tachyzoite, peripheral during intracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "We observed a specific staining of the ER, internal vesicles and cell periphery for TgABCG89, TgABCG84, TgABCG87 and TgABCG96 in intracellular parasites."
  • Microscopy type: light, EM
  • Microscopy method: antibody
  • Strain: RH
  • Gene model mapping comments: blast from DQ099407
  • Localisation record: ER and internal vesicles and cell periphery during intracellular tachyzoite

TGME49_044270 (ABCG87) ABC transporter, putative

Experimental localisation: endoplasmic reticulum during intracellular tachyzoite, cytoplasmic vesicle during intracellular tachyzoite, peripheral during intracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "We observed a specific staining of the ER, internal vesicles and cell periphery for TgABCG89, TgABCG84, TgABCG87 and TgABCG96 in intracellular parasites."
  • Microscopy type: light, EM
  • Microscopy method: antibody to HA tag
  • Strain: RH
  • Gene model mapping comments: blast from DQ099410, gene model inconsistent?
  • Localisation record: ER and internal vesicles and cell periphery during intracellular tachyzoite

TGME49_088920 (ABCG96) ABC transporter, putative

Experimental localisation: endoplasmic reticulum during intracellular tachyzoite, cytoplasmic vesicle during intracellular tachyzoite, peripheral during intracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "We observed a specific staining of the ER, internal vesicles and cell periphery for TgABCG89, TgABCG84, TgABCG87 and TgABCG96 in intracellular parasites."
  • Microscopy type: light, EM
  • Microscopy method: antibody
  • Strain: RH
  • Gene model mapping comments: blast from DQ099412, gene model inconsistent?
  • Localisation record: ER and internal vesicles and cell periphery during intracellular tachyzoite

TGME49_115560 (ABCG77) ABC transporter, putative

Experimental localisation: golgi apparatus during intracellular tachyzoite, parasite plasma membrane during intracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "By comparison, TgABCG77 was more concentrated to the parasite Golgi and the plasma membrane."
  • Microscopy type: light, EM
  • Microscopy method: antibody
  • Strain: RH
  • Gene model mapping comments: blast from DQ099417, gene model inconsistent?
  • Localisation record: golgi and plasma membrane during intracellular tachyzoite

TGME49_047540 (ABCG107) ABC transporter, putative

Experimental localisation: parasitophorous vacuole during intracellular tachyzoite, parasite plasma membrane during intracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Interestingly, TgABCG107 staining was visible in the PV and on the plasma membrane, which may suggest a role for TgABCG107 in lipid movement inside the PV."
  • Microscopy type: light, EM
  • Microscopy method: antibody
  • Strain: RH
  • Gene model mapping comments: blast from DQ099413, gene model inconsistent?
  • Localisation record: PV and plasma membrane during intracellular tachyzoite