ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasion.

Besteiro, S., Michelin, A., Poncet, J., Dubremetz, J. F., Lebrun, M. (2009 Feb, PLoS Pathog)

One of the most conserved features of the invasion process in Apicomplexa parasites is the formation of a moving junction (MJ) between the apex of the parasite and the host cell membrane that moves along the parasite and serves as support to propel it inside the host cell. The MJ was, up to a recent period, completely unknown at the molecular level. Recently, proteins originated from two distinct post-Golgi specialised secretory organelles, the micronemes (for AMA1) and the neck of the rhoptries (for RON2/RON4/RON5 proteins), have been shown to form a complex. AMA1 and RON4 in particular, have been localised to the MJ during invasion. Using biochemical approaches, we have identified RON8 as an additional member of the complex. We also demonstrated that all RON proteins are present at the MJ during invasion. Using metabolic labelling and immunoprecipitation, we showed that RON2 and AMA1 were able to interact in the absence of the other members. We also discovered that all MJ proteins are subjected to proteolytic maturation during trafficking to their respective organelles and that they could associate as non-mature forms in vitro. Finally, whereas AMA1 has previously been shown to be inserted into the parasite membrane upon secretion, we demonstrated, using differential permeabilization and loading of RON-specific antibodies into the host cell, that the RON complex is targeted to the host cell membrane, where RON4/5/8 remain associated with the cytoplasmic face. Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes.

PubMed: 19247437, full text

Localisation information

TGME49_106060 (RON8) hypothetical protein

Experimental localisation: rhoptry neck during intracellular tachyzoite, cytosolic side of host cell membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "RON2, RON4, RON5, and RON8 are all present at the MJ during invasion... we demonstrate that RONs are exported to the host cell membrane, RON/4/5/8 being exposed to the host cell cytosol and RON2 being probably an integral membrane protein that displays a privileged interaction with AMA1."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: Blast from ACK57540.1, gene model inconsistent
  • Localisation record: rhoptry neck during intracellular tachyzoite, cytosolic side of host cell membrane during tachyzoite

TGME49_100100 (RON2) rhoptry neck protein 2

Experimental localisation: moving junction during tachyzoite invasion, rhoptry neck during tachyzoite, host cell plasma membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "RON2, RON4, RON5, and RON8 are all present at the MJ during invasion"
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: inferred from another publication
  • Localisation record: moving junction during tachyzoite invasion, rhoptry neck during tachyzoite, host cell membrane during tachyzoite

TGME49_029010 (RON4) hypothetical protein

Experimental localisation: moving junction during tachyzoite invasion, rhoptry neck during tachyzoite, cytosolic side of host cell membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "RON2, RON4, RON5, and RON8 are all present at the MJ during invasion... we demonstrate that RONs are exported to the host cell membrane, RON/4/5/8 being exposed to the host cell cytosol and RON2 being probably an integral membrane protein that displays a privileged interaction with AMA1."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: inferred from another publication
  • Localisation record: moving junction during tachyzoite invasion, rhoptry neck during tachyzoite, cytosolic side of host cell membrane during tachyzoite

TGME49_111470 (RON5) hypothetical protein

Experimental localisation: moving junction during tachyzoite invasion, rhoptry neck during tachyzoite, cytosolic side of host cell membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "RON2, RON4, RON5, and RON8 are all present at the MJ during invasion... we demonstrate that RONs are exported to the host cell membrane, RON/4/5/8 being exposed to the host cell cytosol and RON2 being probably an integral membrane protein that displays a privileged interaction with AMA1."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: Taken directly from 19247437
  • Localisation record: moving junction during tachyzoite invasion, rhoptry neck during tachyzoite, cytosolic side of host cell membrane during tachyzoite

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: parasite plasma membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "The extracellular portion of the tachyzoites was labelled with anti-SAG1 (green), and, then, after permeabilization of the cells with saponin, the ring of the MJ was revealed by addition of the conjugate (red)."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during tachyzoite

TGME49_086450 (GRA5) dense granule protein 5 precursor

Experimental localisation: parasitophorous vacuole membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: ""
  • Microscopy type: Light
  • Microscopy method: HA tag, monoclonal antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PVM during tachyzoite