ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Plasmodium falciparum secretory pathway: characterization of PfStx1, a plasma membrane Qa-SNARE.

Parish, L. A., Rayner, J. C. (2009 Apr, Mol Biochem Parasitol)

SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) play a central role in regulating and facilitating vesicular traffic in eukaryotic cells. While SNAREs have been well characterized in other eukaryotes, little is known about their role in the unique protein trafficking pathways in Plasmodium falciparum. We have identified seven Qa-SNAREs in the P. falciparum genome and confirmed the gene structure of all seven, which in one case differs from the predicted structure in the database. Based on comprehensive sequence alignments we made predictions for the intracellular locations of all seven P. falciparum Qa-SNAREs, and confirmed the predicted location for one Qa-SNARE, PfStx1, which is most closely related to other eukaryotic plasma membrane Qa-SNAREs such as syntaxin 1. This is the first identified trafficking component localized proximal to the P. falciparum plasma membrane.

PubMed: 19103232, full text

Localisation information

PFB0480w (Stx1) syntaxin, Qa-SNARE family

Experimental localisation: proximal to plasma membrane during schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Fig. 2. PfStx1 is localized proximal to the P. falciparum plasma membrane."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: 3D7
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: proximal to plasma membrane during schizont

PFI0875w (grp, Hsp70-2, BiP) heat shock protein 70

Experimental localisation: endoplasmic reticulum during schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "PfSyn1 (green) again has a peripheral location that is distinct from the ER marker PfBiP (in red) (C). "
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: 3D7
  • Gene model mapping comments: inferred from another publication
  • Localisation record: ER during schizont

PF10_0345 (MSP3.1, MSP3) merozoite surface protein 3

Experimental localisation: parasite plasma membrane during schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "PfStx1 (red) co-localizes with PfMSP3 (in green), a plasma membrane marker in schizont stage parasites (D), confirming its predicted location proximal to the plasma membrane."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: 3D7
  • Gene model mapping comments: inferred from another publication
  • Localisation record: plasma membrane during schizont