ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Deletion of the Plasmodium falciparum merozoite surface protein 7 gene impairs parasite invasion of erythrocytes.

Kadekoppala, M., O'Donnell, R. A., Grainger, M., Crabb, B. S., Holder, A. A. (2008 Dec, Eukaryot Cell)

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria parasite. In Plasmodium falciparum, merozoite surface proteins include several glycosylphosphatidyl inositol-anchored proteins and peripheral proteins attached to the membrane through protein-protein interactions. The most abundant of these proteins is the merozoite surface protein 1 (MSP1) complex, encoded by at least three genes: msp1, msp6, and msp7. The msp7 gene is part of a six-member multigene family in Plasmodium falciparum. We have disrupted msp7 in the Plasmodium falciparum D10 parasite, as confirmed by Southern hybridization. Immunoblot and indirect immunofluorescence analyses confirmed the MSP7 null phenotype of D10DeltaMSP7 parasites. The synthesis, distribution, and processing of MSP1 were not affected in this parasite line. The level of expression and cellular distribution of the proteins MSP1, MSP3, MSP6, MSP9, and SERA5 remained comparable to those for the parental line. Furthermore, no significant change in the expression of MSP7-related proteins, except for that of MSRP5, was detected at the transcriptional level. The lack of MSP7 was not lethal at the asexual blood stage, but it did impair invasion of erythrocytes by merozoites to a significant degree. Despite this reduction in efficiency, D10DeltaMSP7 parasites did not show any obvious preference for alternate pathways of invasion.

PubMed: 18820076, full text

Localisation information

PFI1475w (MSP1, MSP-1, msp1 unprocessed, msp1, Msp-1 19, MSP1 19) merozoite surface protein 1

Experimental localisation: parasite plasma membrane during late schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Absence of MSP7 does not alter expression and distribution of several other peripheral merozoite surface proteins. In addition to MSP7, several other proteins have been reported to interact with the MSP1 complex through protein-protein interactions. We investigated expression and distribution of MSP3, MSP6, and MSP9 (ABRA) and a parasitophorous vacuolar protein, SERA-5, in order to examine the consequence of altering the nature of the MSP1 complex."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: D10
  • Gene model mapping comments: annotation matches
  • Localisation record: surface during late schizont

PF10_0345 (MSP3.1, MSP3) merozoite surface protein 3

Experimental localisation: parasite plasma membrane during late schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Absence of MSP7 does not alter expression and distribution of several other peripheral merozoite surface proteins. In addition to MSP7, several other proteins have been reported to interact with the MSP1 complex through protein-protein interactions. We investigated expression and distribution of MSP3, MSP6, and MSP9 (ABRA) and a parasitophorous vacuolar protein, SERA-5, in order to examine the consequence of altering the nature of the MSP1 complex."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: D10
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during late schizont

PF10_0346 (MSP-6 36, MSP3.2, MSP6) merozoite surface protein 6

Experimental localisation: parasite plasma membrane during late schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Absence of MSP7 does not alter expression and distribution of several other peripheral merozoite surface proteins. In addition to MSP7, several other proteins have been reported to interact with the MSP1 complex through protein-protein interactions. We investigated expression and distribution of MSP3, MSP6, and MSP9 (ABRA) and a parasitophorous vacuolar protein, SERA-5, in order to examine the consequence of altering the nature of the MSP1 complex."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: D10
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during late schizont

PFL1385c (ABRA, MSP9, MSP-9) merozoite surface protein 9

Experimental localisation: parasite plasma membrane during late schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Absence of MSP7 does not alter expression and distribution of several other peripheral merozoite surface proteins. In addition to MSP7, several other proteins have been reported to interact with the MSP1 complex through protein-protein interactions. We investigated expression and distribution of MSP3, MSP6, and MSP9 (ABRA) and a parasitophorous vacuolar protein, SERA-5, in order to examine the consequence of altering the nature of the MSP1 complex."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: D10
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during late schizont

PFB0340c (SERA5, SERP, p126, SERA, pl26) serine repeat antigen 5

Experimental localisation: parasitophorous vacuole during late schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Absence of MSP7 does not alter expression and distribution of several other peripheral merozoite surface proteins. In addition to MSP7, several other proteins have been reported to interact with the MSP1 complex through protein-protein interactions. We investigated expression and distribution of MSP3, MSP6, and MSP9 (ABRA) and a parasitophorous vacuolar protein, SERA-5, in order to examine the consequence of altering the nature of the MSP1 complex."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: D10
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during late schizont