ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa
|version 3 (curated until May 28, 2011)|
Identification of trafficking determinants for polytopic rhomboid proteases in Toxoplasma gondii.
Sheiner, L., Dowse, T. J., Soldati-Favre, D. (2008 May, Traffic)
Rhomboids (ROMs) constitute a family of polytopic serine proteases conserved throughout evolution. The obligate intracellular parasite Toxoplasma gondii possesses six genes coding for ROM-like proteases that are targeted to distinct subcellular compartments: TgROM1 localizes to regulated secretory organelles, micronemes, TgROM2 is present in the Golgi, while TgROM4 and TgROM5 are found in the pellicle of the parasite. The targeting mechanism/s of ROM proteins is an aspect that has not yet been assessed. The existence of TgROM family members localized to different subcellular compartments provides a convenient system to study their sorting mechanisms in a genetically tractable organism that possesses an elaborate secretory pathway and conserved trafficking machineries. In this study, we experimentally established the topology of TgROM1 and TgROM4 at the plasma membrane and applied domain-exchange and site-directed mutagenesis approaches to identify critical sorting determinants on the N-terminal cytosolic domains of TgROM2 and TgROM1 that confer their Golgi and post-Golgi localizations, respectively.
TGME49_068590 (ROM4) rhomboid-like protease 4Experimental localisation: parasite plasma membrane during intracellular tachyzoite, parasite plasma membrane during extracellular tachyzoite, not inner membrane complex during intracellular tachyzoite, not inner membrane complex during extracellular tachyzoite
TGME49_063290 (ROM2) rhomboid-like protease TgROM2Experimental localisation: golgi apparatus during tachyzoite, trans-golgi network during tachyzoite, parasite plasma membrane during tachyzoite
TGME49_000290 (ROM1) rhomboid family domain-containing proteinExperimental localisation: endoplasmic reticulum during intracellular tachyzoite, golgi apparatus during intracellular tachyzoite, rhoptry during intracellular tachyzoite, post golgi compartment during intracellular tachyzoite
TGME49_008030 (MIC4, MIC 4) microneme protein MIC4Experimental localisation: microneme during tachyzoite