ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Molecular and biochemical characterization of Toxoplasma gondii beta-hydroxyacyl-acyl carrier protein dehydratase (FABZ).

Dautu, G., Ueno, A., Munyaka, B., Carmen, G., Makino, S., Kobayashi, Y., Igarashi, M. (2008 May, Parasitol Res)

Toxoplasma gondii, unlike its mammalian host, utilizes a type II fatty acid biosynthesis pathway in which the steps of fatty acid biosynthesis are catalyzed by independent enzymes. Due to this difference, the enzymes of this pathway are good targets for the development of new therapeutic drugs directed against toxoplasmosis. In this report, we show by using reverse transcription-polymerase chain reaction analysis that beta-Hydroxyacyl-acyl carrier protein dehydratase (TgFABZ) is expressed both in tachyzoites and bradyzoites. Indirect immunofluorescence antibody test further shows the localization of TgFABZ protein in the apicoplast of both tachyzoites and bradyzoites. Enzyme dynamic analysis shows that the purified recombinant TgFABZ protein is soluble and active. The Km value of the enzyme for its substrate analog crotonoyl-CoA was estimated to be 82.57 +/- 10 microM.

PubMed: 18274777, full text

Localisation information

TGME49_121570 (FABZ) (3R)-hydroxymyristoyl ACP dehydrase, putative

Experimental localisation: apicoplast during tachyzoite, apicoplast during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Indirect immunofluorescence antibody test further shows the localization of TgFABZ protein in the apicoplast of both tachyzoites and bradyzoites."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: Beverley
  • Gene model mapping comments: annotation searched and FabZ is the proposed gene name according to ToxoDB 5.0
  • Localisation record: apicoplast during tachyzoite and bradyzoite

TGME49_064080 (ACP) acyl carrier protein

Experimental localisation: apicoplast during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "To prove that TgFABZ localizes in the apicoplast, the TgFABZ was co-stained with a known apicoplast marker TgACP."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: Beverley
  • Gene model mapping comments: inferred from another publication
  • Localisation record: apicoplast during tachyzoite

TGME49_059020 (BAG1) bradyzoite antigen, putative

Experimental localisation: parasite plasma membrane during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Furthermore, brain tissue sections from infected mice incubated with polyclonal anti- TgFABZ antibody showed localized staining in the T. gondii (Fig. 3c). On the other hand, brain sections incubated with polyclonal anti-BAG1 antibody (used as positive control) stained the T. gondii cyst uniformly (Fig. 3e)."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: Beverley
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during bradyzoite