ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Differential targeting of dense granule proteins in the parasitophorous vacuole of Toxoplasma gondii.

Achbarou, A., Mercereau-Puijalon, O., Sadak, A., Fortier, B., Leriche, M. A., Camus, D., Dubremetz, J. F. (1991 Dec, Parasitology)

The biosynthesis and fate of 4 different dense granule proteins of Toxoplasma gondii were studied with 3 monoclonal antibodies raised against tachyzoites and 1 polyclonal antibody raised against a recombinant protein. These proteins have the following molecular weights: 27 kDa (GRA 1), 28 kDa (GRA 2), 30 kDa (GRA 3) and 40 kDa (GRA 4). All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane. Therefore, dense granule contents undergo differential targeting when exocytosed in the parasitophorous vacuole. Metabolic labelling and immunoprecipitation showed that GRA 2 and GRA 3 were processed from lower molecular weight precursors, and that GRA 2 and GRA 4 incorporated [3H] glucosamine and are thus likely to be glycosylated.

PubMed: 1780169, full text

Localisation information

TGME49_070250 (GRA1) dense granule protein 1 / major antigenp24

Experimental localisation: dense granule during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane."
  • Microscopy type: EM
  • Microscopy method: antibody directly to protein
  • Strain:
  • Gene model mapping comments: Blast from orthologue AY223805, annotation matches
  • Localisation record: dense granule during tachyzoite
  • Comment: Couldn't find Full Text – strain not recorded in abstract

TGME49_027620 (GRA2) 28 kDa antigen

Experimental localisation: dense granule during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane."
  • Microscopy type: EM
  • Microscopy method: antibody directly to protein
  • Strain:
  • Gene model mapping comments: Only annotation match
  • Localisation record: dense granule during tachyzoite
  • Comment: Couldn't find Full Text – strain not recorded in abstract

TGME49_027280 (GRA3) dense granule protein 3

Experimental localisation: dense granule during tachyzoite, parasitophorous vacuole membrane during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane."
  • Microscopy type: EM
  • Microscopy method: antibody directly to protein
  • Strain:
  • Gene model mapping comments: Only annotation match
  • Localisation record: dense granule and PVM during tachyzoite
  • Comment: Couldn't find Full Text – strain not recorded in abstract

TGME49_110780 (GRA4) hypothetical protein

Experimental localisation: dense granule during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane."
  • Microscopy type: EM
  • Microscopy method: antibody directly to protein
  • Strain:
  • Gene model mapping comments: Blast from AF161267.1, annotation matches
  • Localisation record: dense granule during tachyzoite
  • Comment: Couldn't find Full Text – strain not recorded in abstract