ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Protective properties and surface localization of Plasmodium falciparum enolase.

Pal-Bhowmick, I., Mehta, M., Coppens, I., Sharma, S., Jarori, G. K. (2007 Nov, Infect Immun)

The enolase protein of the human malarial parasite Plasmodium falciparum has recently been characterized. Apart from its glycolytic function, enolase has also been shown to possess antigenic properties and to be present on the cell wall of certain invasive organisms, such as Candida albicans. In order to assess whether enolase of P. falciparum is also antigenic, sera from residents of a region of Eastern India where malaria is endemic were tested against the recombinant P. falciparum enolase (r-Pfen) protein. About 96% of immune adult sera samples reacted with r-Pfen over and above the seronegative controls. Rabbit anti-r-Pfen antibodies inhibited the growth of in vitro cultures of P. falciparum. Mice immunized with r-Pfen showed protection against a challenge with the 17XL lethal strain of the mouse malarial parasite Plasmodium yoelii. The antibodies raised against r-Pfen were specific for Plasmodium and did not react to the host tissues. Immunofluorescence as well as electron microscopic examinations revealed localization of the enolase protein on the merozoite cell surface. These observations establish malaria enolase to be a potential protective antigen.

PubMed: 17785475, full text

Localisation information

PF10_0155 (enolase) enolase

Experimental localisation: parasite plasma membrane during merozoite, parasite plasma membrane during schizont, cytoplasm during merozoite, cytoplasm during schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "An indirect IFA showed that in addition to the cytoplasmic presence of enolase, the typical beehive pattern of a surface protein was also observed when P. falciparum schizonts were stained with anti-enolase antibodies, and there was significant colocalization of enolase with MSP-1 protein (Fig. 2D). These results support the view that enolase is localized on the surface of P. falciparum merozoites."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly against protein
  • Strain: 3D7
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface and cytoplasm during merozoite and schizont

PFI1475w (MSP1, MSP-1, msp1 unprocessed, msp1, Msp-1 19, MSP1 19) merozoite surface protein 1

Experimental localisation: parasite plasma membrane during merozoite
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Therefore, we studied the localization of enolase in mature segmented schizonts of P. falciparum along with a known merozoite surface marker, merozoite surface protein 1 (MSP-1) (Fig. 2D)."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: 3D7
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during merozoite