ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa
|version 3 (curated until May 28, 2011)|
A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii.
Taylor, S., Barragan, A., Su, C., Fux, B., Fentress, S. J., Tang, K., Beatty, W. L., Hajj, H. E., Jerome, M., Behnke, M. S., White, M., Wootton, J. C., Sibley, L. D. (2006 Dec 15, Science)
Toxoplasma gondii strains differ dramatically in virulence despite being genetically very similar. Genetic mapping revealed two closely adjacent quantitative trait loci on parasite chromosome VIIa that control the extreme virulence of the type I lineage. Positional cloning identified the candidate virulence gene ROP18, a highly polymorphic serine-threonine kinase that was secreted into the host cell during parasite invasion. Transfection of the virulent ROP18 allele into a nonpathogenic type III strain increased growth and enhanced mortality by 4 to 5 logs. These attributes of ROP18 required kinase activity, which revealed that secretion of effectors is a major component of parasite virulence.
TGME49_005250 (ROP18) Rhoptry kinase family protein ROP18Experimental localisation: parasitophorous vacuole during early intracellular tachyzoite, cytoplasmic vesicle during early intracellular tachyzoite, rhoptry during late intracellular tachyzoite
TGME49_109590 (ROP1) rhoptry protein, putativeExperimental localisation: rhoptry