ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

The Plasmodium vivax homolog of the ookinete adhesive micronemal protein, CTRP.

Kaneko, O., Templeton, T. J., Iriko, H., Tachibana, M., Otsuki, H., Takeo, S., Sattabongkot, J., Torii, M., Tsuboi, T. (2006 Sep, Parasitol Int)

The Plasmodium circumsporozoite protein/thrombospondin-related anonymous protein-related protein (CTRP) is expressed at the mosquito midgut ookinete stage and is considered to be a transmission-blocking vaccine candidate. CTRP is composed of multiple von Willebrand factor A (vWA) and thrombospondin type 1 domains in the extracellular portion of the molecule, and a short acidic cytoplasmic domain that interacts with the actomyosin machinery. As a means to predict functionally relevant domains within CTRP we determined the nucleotide sequences of CTRP from the Plasmodium vivax Sall and the Plasmodium yoelii 17XL strains and characterized the conservation of domain architectures and motifs across Plasmodium genera. Sequence alignments indicate that the CTRP 1st to 4th vWA domains exhibit greater conservation, and thereby are perhaps functionally more important than the 5th and 6th domains. This point should be considered for the development of a transmission-blocking vaccine that includes CTRP recombinant subunit. To complement previous cellular studies on CTRP, we further determined the expression and cellular localization of CTRP protein in P. vivax and P. yoelii.

PubMed: 16822707, full text

Localisation information

PY04858 (CTRP) ookinete protein-related

Experimental localisation: apical during ookinete
  • Species: Plasmodium yoelii
  • Quote inferring localisation: "we determined the PvCTRP and PyCTRP nucleotide sequences and show protein expression in ookinete stages that is localized to the apical region."
  • Microscopy type: Light
  • Microscopy method: antibody to orthologue
  • Strain: 17XL
  • Gene model mapping comments: Blast from AB247368
  • Localisation record: apical during ookinete

PVX_095475 (CTRP) CTRP adhesive protein (invasive stage), putative

Experimental localisation: apical during ookinete
  • Species: Plasmodium vivax
  • Quote inferring localisation: "we determined the PvCTRP and PyCTRP nucleotide sequences and show protein expression in ookinete stages that is localized to the apical region."
  • Microscopy type: Light
  • Microscopy method: antibody to orthologue
  • Strain: Plasmodium vivax "field isolate"
  • Gene model mapping comments: Blast from AB247369
  • Localisation record: apical during ookinete
  • Comment: Blast shows possibly incorrect gene model

AB247370 (CTRP) gi|110681633|dbj|AB247370.1| Plasmodium gallinaceum pgctrp gene for circumsporozoite protein/thrombospondin-related anonymous protein-related protein, partial cds, strain:7A

Experimental localisation: microneme during ookinete, sub-pellicular region at the anterior during ookinete
  • Species: Plasmodium gallinaceum
  • Quote inferring localisation: "The immuno-gold particles associated with micronemes and the subpellicular region at the anterior portion. "
  • Microscopy type: EM
  • Microscopy method: polyclonal antibody to homologue
  • Strain:
  • Gene model mapping comments: AB247370 taken directly from publication
  • Localisation record: microneme and sub-pellicular region at the anterior portion during ookinete
  • Comment: strain information not found