ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Metabolic maps and functions of the Plasmodium mitochondrion.

van Dooren, G. G., Stimmler, L. M., McFadden, G. I. (2006 Jul, FEMS Microbiol Rev)

The mitochondrion of Plasmodium species is a validated drug target. However, very little is known about the functions of this organelle. In this review, we utilize data available from the Plasmodium falciparum genome sequencing project to piece together putative metabolic pathways that occur in the parasite, comparing this with the existing biochemical and cell biological knowledge. The Plasmodium mitochondrion contains both conserved and unusual features, including an active electron transport chain and many of the necessary enzymes for coenzyme Q and iron-sulphur cluster biosynthesis. It also plays an important role in pyrimidine metabolism. The mitochondrion participates in an unusual hybrid haem biosynthesis pathway, with enzymes localizing in both the mitochondrion and plastid organelles. The function of the tricarboxylic acid cycle in the mitochondrion is unclear. We discuss directions for future research into this fascinating, yet enigmatic, organelle.

PubMed: 16774588, full text

Localisation information

MAL13P1.326 (FC, HemH) ferrochelatase

Experimental localisation: mitochondrion, not apicoplast
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "A recent study, however, presented immunofluorescence data suggesting Plasmodium HemH is in fact apicoplast localized (Varadharajan et al., 2004). To test this surprising result, we fused HemH to a short epitope tag and expressed the resulting fusion protein in P. falciparum cells. Using immunofluorescence assays to co-label epitope-tagged HemH with either an apicoplast or a mitochondrial marker clearly demonstrated the mitochondrial localization of this enzyme"
  • Microscopy type: Light
  • Microscopy method: epitope tag
  • Strain:
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: mitochondria and not apicoplast
  • Comment: Unable to find strain in the paper

PFE1230c (Tom22) mitochondrial import receptor subunit tom22, putative

Experimental localisation: mitochondrion
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "PfTom22 localizes to the mitochondrion of P. falciparum"
  • Microscopy type: Light
  • Microscopy method: N terminal HA tag
  • Strain:
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: Mitochondria
  • Comment: Unable to find strain in the paper

PF08_0031 (OMT) oxoglutarate/malate translocator protein, putative

Experimental localisation: mitochondrion
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "The oxoglutarate/malate transporter homologue of P. falciparum (PfOMT) localizes to the mitochondrion of P. falciparum."
  • Microscopy type: Light
  • Microscopy method: C terminal GFP tag
  • Strain:
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: Mitochondria
  • Comment: Unable to find strain in the paper

PFI0735c (NDH) type II NADH:quinone oxidoreductase

Experimental localisation: mitochondrion during gametocyte, mitochondrion during ring, mitochondrion during trophozoite, mitochondrion during schizont, mitochondrion during merozoite, cytosol during ring, cytosol during trophozoite, cytosol during schizont, cytosol during merozoite
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "The single-subunit NAD(P)H dehydrogenase homologue of P. falciparum (PfNDH) localizes to the mitochondrion... In asexual stages, when protein expression levels are probably higher, a considerable portion of PfNDHL-GFP also localizes to the cytosol, suggesting that import of this fusion protein is somewhat inefficient."
  • Microscopy type: Light
  • Microscopy method: N terminal GFP tag
  • Strain:
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: Mitochondria during gametocyte, mitochondria and cytosol during asexual stages
  • Comment: Unable to find strain in the paper

PFF0370w (Coq2) para-hydroxybenzoate--polyprenyltransferase (4- hydroxybenzoate octaprenyltransferase), putative

Experimental localisation: mitochondrion
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "The coenzyme Q biosynthesis protein Coq2 homologue of P. falciparum (PfCoq2) localizes to the mitochondrion"
  • Microscopy type: Light
  • Microscopy method: N terminal GFP tag
  • Strain:
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: Mitochondria
  • Comment: Unable to find strain in the paper