ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage.

Yano, K., Komaki-Yasuda, K., Kobayashi, T., Takemae, H., Kita, K., Kano, S., Kawazu, S. (2005 Mar, Parasitol Int)

mRNA and protein expression profiles for three peroxiredoxins (PfTPx-1, PfTPx-2 and Pf1-Cys-Prx) and a thioredoxin (PfTrx-1) of Plasmodium falciparum during the erythrocytic stage were examined by real-time quantitative reverse transcription-PCR (RT-PCR), Western blotting and confocal laser scanning microscopy. PfTPx-1 was expressed constitutively in the parasite cytoplasm throughout the erythrocytic stage, suggesting a housekeeping role of this enzyme for control of intercellular reactive oxygen species (ROS) in the parasite. Pf1-Cys-Prx showed elevated expression during the trophozoite and early schizont stages in the parasite cytoplasm, and this profile suggested that this peroxiredoxin (Prx) detoxifies metabolism-derived ROS such as those released from heme iron. The other 2-Cys Prx, PfTPx-2, was detected in mitochondria and was expressed in both the trophozoite and schizont stages. Detection of the Prx in mitochondria is consistent with recent reports of the existence of a respiratory chain, which produces ROS, in the mitochondria of P. falciparum. PfTrx-1 showed elevated expression during the trophozoite and schizont stages in the parasite cytoplasm. Finally, expression of these antioxidant protein genes is most likely regulated at the transcriptional level because their mRNA and protein expression profiles overlapped.

PubMed: 15710548, full text

Localisation information

PF14_0368 (TPx-1) thioredoxin peroxidase 1

Experimental localisation: cytoplasm during ring, cytoplasm during trophozoite, cytoplasm during schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Immunofluorescence microscopy of P. falciparum erythrocytic-stage parasites revealed that PfTPx-1 was expressed in the parasite cytoplasm during the ring, trophozoite and schizont stages."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: FCR-3
  • Gene model mapping comments: blast from primers TCT GAA ATG GCA TCA TAT GTA GGA and CGG AAT TCT TAC AAC TTT GAT AAA TAT T
  • Localisation record: cytoplasm during ring and troph and schizont

PF08_0131 (1-Cys-Prx) 1-cys peroxiredoxin

Experimental localisation: cytoplasm during trophozoite, ring, late schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "In contrast, significant staining of Pf1-Cys-Prx was detected in the trophozoites in the cytoplasm of the parasite"
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: FCR-3
  • Gene model mapping comments: blast from primers TCG TGA GAT TCT TTG GAG TT and ATG GCT TAC CAT TTA GGA GCT AC
  • Localisation record: cytoplasm during trophozoite, weak during ring and late schizont

PF14_0545 (Trx-1) thioredoxin, putative

Experimental localisation: cytoplasm during after trophozoite
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Significant expression of PfTrx-1 was observed after the trophozoite stage, but expression was weak during the ring stage"
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: FCR-3
  • Gene model mapping comments: Taken directly from 15387810, annotation matches
  • Localisation record: cytoplasm during after trophozoite

PFL0725w (pftrx-px2, TPx-2) thioredoxin peroxidase 2

Experimental localisation: mitochondrion during trophozoite, mitochondrion during schizont
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Double staining of parasite cells with anti-PfTPx-2 serum and Mito Tracker® revealed that PfTPx-2 was present in mitochondria (Fig. 4A–F)."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: FCR-3
  • Gene model mapping comments: blast from AF225978, annotation matches
  • Localisation record: mitochondria during trophozoite and schizont