ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Merozoite surface proteins 4 and 5 of Plasmodium knowlesi have differing cellular localisation and association with lipid rafts.

Black, C. G., Wang, L., Topolska, A. E., Finkelstein, D. I., Horne, M. K., Thomas, A. W., Mohandas, N., Coppel, R. L. (2004 Nov, Mol Biochem Parasitol)

PubMed: 15500926, full text

Localisation information

PKH_041300 (MSP-4, MSP4) merozoite surface protein, putative

Experimental localisation: surface during merozoite
  • Species: Plasmodium knowlesi
  • Quote inferring localisation: "As shown in Fig. 2B, expression of PkMSP4 was localised to the merozoite surface as was to be expected based on previous results with antibodies to PfMSP4 and PvMSP4"
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: H
  • Gene model mapping comments: blast from AY573057, annotation matches
  • Localisation record: surface during merozoite

PKH_041310 (MSP-5, MSP5) merozoite surface protein 5

Experimental localisation: apical during merozoite
  • Species: Plasmodium knowlesi
  • Quote inferring localisation: "As observed for PvMSP5, the pattern of fluorescent reactivity with antiserum to PkMSP5 was not consistent with the ‘bunch of grapes’ surface rim pattern typically seen with antibodies to other MSPs. Instead, the pattern seen was a single large dot or spot of fluorescence at one end of the merozoite...MSP5 was associated with an apical organelle approximate to the micronemes or over the polar cap "
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: H
  • Gene model mapping comments: blast from AY573058, annotation matches
  • Localisation record: apical during merozoite