ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

GRA9, a new Toxoplasma gondii dense granule protein associated with the intravacuolar network of tubular membranes.

Adjogble, K. D., Mercier, C., Dubremetz, J. F., Hucke, C., Mackenzie, C. R., Cesbron-Delauw, M. F., Daubener, W. (2004 Oct, Int J Parasitol)

Important components of the parasitophorous vacuole in which the intracellular protozoan parasite Toxoplasma gondii develops, comprise proteins secreted from apicomplexan specific secretory organelles named the dense granules. Here, we confirm by immunofluorescence and by cryo-electron microscopy that the recently isolated B10 protein (318 amino acids, 41kDa) is a new dense granule protein that should now be referred to as GRA9. Within the vacuolar compartment, GRA9, like GRA2, GRA4 and GRA6, associates with the network of tubular membranes connected to the parasitophorous vacuole delimiting membrane. Like the other GRA proteins, GRA9 is secreted into the vacuole from the anterior end of the parasite. However, unlike GRA2 or GRA6, GRA9 does not transit by the posterior invaginated pocket of the parasite where the network first assembles. Within the dense granules, GRA9 exists in both a soluble and an insoluble state. Like the other GRA proteins, GRA9 is secreted as a soluble form only and like most of the GRA proteins, two forms of GRA9 of the similar molecular weight are detected within the vacuolar space: a soluble form and a membrane associated form. The dual properties of GRA9 are not only ascribed by the presence of amphipathic and hydrophobic alpha-helices but also by the fact that the protein is mainly hydrophilic.

PubMed: 15491588, full text

Localisation information

TGME49_051540 (GRA9) GRA9 protein, putative

Experimental localisation: dense granule during tachyzoite, parasitophorous vacuole during tachyzoite, membranous parasitophorous vacuole tubules during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Here, we confirm by immunofluorescence and by cryo-electron microscopy that the recently isolated B10 protein (318 amino acids, 41┬ákDa) is a new dense granule protein that should now be referred to as GRA9."
  • Microscopy type: Light and EM
  • Microscopy method: antibody directly to protein
  • Strain: BK
  • Gene model mapping comments: Blast from AJ223585
  • Localisation record: dense granule and PV and membranous PV tubules during tachyzoite