ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Localization of a 56-kDa antigen that is present in multiple developmental stages of Neospora caninum.

Jenkins, M., Soares, R., Murphy, C., Hemphill, A., O'Handley, R., Dubey, J. P. (2004 Jun, J Parasitol)

The purpose of the present study was to characterize the intracellular distribution of a native Neospora caninum 56-kDa protein that is recognized by sera from N. caninum-infected dairy cattle. The complementary DNA coding for this protein was expressed in Escherichia coli as a polyHis fusion protein to which antiserum was prepared and used to localize the antigen in N. caninum tachyzoites and bradyzoites. By sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting, antirecombinant Nc56 serum recognized a major 56-kDa protein and 2 minor (43 and 39 kDa) proteins of N. caninum tachyzoites. Antiserum to recombinant 56-kDa protein showed this antigen to be present in both N. caninum tachyzoites and bradyzoites/cysts as detected by immunofluorescence staining. Immunoelectron microscopy revealed the 56-kDa antigen to be present in the apical end of both tachyzoites and bradyzoites and possibly extracellularly secreted by tachyzoites.

PubMed: 15270121, full text

Localisation information

NCLIV_028990 (56-kDa antigen) hypothetical protein

Experimental localisation: apical during bradyzoite, apical during tachyzoite
  • Species: Neospora caninum
  • Quote inferring localisation: "Immunoelectron microscopy revealed the 56-kDa antigen to be present in the apical end of both tachyzoites and bradyzoites and possibly extracellularly secreted by tachyzoites."
  • Microscopy type: light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from AAR02100, annotation matches
  • Localisation record: apical during bradyzoite and tachyzoite