ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondii.

Gaskins, E., Gilk, S., DeVore, N., Mann, T., Ward, G., Beckers, C. (2004 May 10, J Cell Biol)

Apicomplexan parasites exhibit a unique form of substrate-dependent motility, gliding motility, which is essential during their invasion of host cells and during their spread between host cells. This process is dependent on actin filaments and myosin that are both located between the plasma membrane and two underlying membranes of the inner membrane complex. We have identified a protein complex in the apicomplexan parasite Toxoplasma gondii that contains the class XIV myosin required for gliding motility, TgMyoA, its associated light chain, TgMLC1, and two novel proteins, TgGAP45 and TgGAP50. We have localized this complex to the inner membrane complex of Toxoplasma, where it is anchored in the membrane by TgGAP50, an integral membrane glycoprotein. Assembly of the protein complex is spatially controlled and occurs in two stages. These results provide the first molecular description of an integral membrane protein as a specific receptor for a myosin motor, and further our understanding of the motile apparatus underlying gliding motility in apicomplexan parasites.

PubMed: 15123738, full text

Localisation information

TGME49_035470 (MyoA) myosin A, putative

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: ""
  • Microscopy type: Light
  • Microscopy method: HA tag
  • Strain: RH HXGPRT–
  • Gene model mapping comments: annotation matches, community comments match
  • Localisation record: inner membrane complex
  • Comment: Protein complex: myosin-A, GAP45, GAP50

TGME49_023940 (GAP45) hypothetical protein

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "In untreated cells, the plasma membrane protein SAG1 is evenly distributed over the parasite circumference, whereas TgGAP45 distribution reveals an anterior gap (arrows) consistent with localization to the inner membrane complex."
  • Microscopy type: Light, EM
  • Microscopy method: monospecific antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: Blast from AAP41369
  • Localisation record: inner membrane complex
  • Comment: Protein complex: myosin-A, GAP45, GAP50

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: parasite plasma membrane
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "In untreated cells, the plasma membrane protein SAG1 is evenly distributed over the parasite circumference, whereas TgGAP45 distribution reveals an anterior gap (arrows) consistent with localization to the inner membrane complex."
  • Microscopy type: Light
  • Microscopy method:
  • Strain: RH HXGPRT–
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface
  • Comment: localisation method not discussed

TGME49_019320 (GAP50) acid phosphatase, putative

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "In untreated cells, the plasma membrane protein SAG1 is evenly distributed over the parasite circumference, whereas an anterior gap in TgGAP50-YFP distribution (arrows) is consistent with localization to the inner membrane complex. In toxin-treated parasites, the plasma membrane is markedly distended, whereas the distribution of TgGAP50-YFP is not affected, confirming its association with the inner membrane complex"
  • Microscopy type: Light
  • Microscopy method: YFP tag
  • Strain: RH HXGPRT–
  • Gene model mapping comments: Blast from AY587763
  • Localisation record: inner membrane complex
  • Comment: Protein complex: myosin-A, GAP45, GAP50

TGME49_057680 (MLC1) myosin light chain TgMLC1

Experimental localisation: inner membrane complex
  • Species: Toxoplasma gondii
  • Quote inferring localisation: ""
  • Microscopy type: Light
  • Microscopy method: monospecific antibody directly to protein
  • Strain: RH HXGPRT–
  • Gene model mapping comments: inferred from another publication
  • Localisation record: inner membrane complex