ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Use of molecular and ultrastructural markers to evaluate stage conversion of Toxoplasma gondii in both the intermediate and definitive host.

Ferguson, D. J. (2004 Mar 9, Int J Parasitol)

Toxoplasma gondii has a complex life cycle involving definite (cat) and intermediate (all warm blooded animals) hosts. This gives rise to four infectious forms each of which has a distinctive biological role. Two (tachyzoite and merozoite) are involved in propagation within a host and two (bradyzoite and sporozoite) are involved in transmission to new hosts. The various forms can be identified by their structure, host parasite relationship and distinctive developmental processes. In the present in vivo study, the various stages have been evaluated by electron microscopy and immunocytochemistry using a panel of molecular markers relating to surface and cytoplasmic molecules, metabolic iso-enzymes and secreted proteins that can differentiate between tachyzoite, bradyzoite and coccidian development. Tachyzoites were characterised as being positive for surface antigen 1, enolase isoenzyme 2, lactic dehydrogenase isoenzyme 1 and negative for bradyzoite antigen 1. In contrast, bradyzoites were negative for SAG1 but positive for BAG1, ENO1 and LDH2. When stage conversion was followed in brain lesion at 10 and 15 days post-infection, tachyzoites were predominant but a number of single intermediate organisms displaying tachyzoite and certain bradyzoite markers were observed. At later time points, small groups of organisms displaying only bradyzoite markers were also present. A number (9) of dense granule proteins (GRA1-8, NTPase) have also been identified in both tachyzoites and bradyzoites but there were differences in their location during parasite development. All the dense granule proteins extensively label the parasitophorous vacuole during tachyzoite development. In contrast the tissue cyst wall displays variable staining for the dense granule proteins, which also expresses an additional unique cyst wall protein. The molecular differences could be identified at the single cell stage consistent with conversion occurring at the time of entry into a new cell. These molecular differences were reflected in the structural differences in the parasitophorous vacuoles observed by electron microscopy. Stage conversion to enteric (coccidian) development was limited to the enterocytes of the cat small intestine. Although no specific markers were available, this form of development can be identified by the absence of specific tachyzoite (SAG1) and bradyzoite (BAG1) markers although the isoenzymes ENO2 and LHD1 were expressed. There was also a significant difference in the expression of the dense granule proteins. The coccidian stages and merozoites only expressed two (GRA7 and NTPase) of the nine dense granule proteins and this was reflected in significant differences in the structure of the parasitophorous vacuole. The coccidian stages also undergo conversion from asexual to sexual development. The mechanism controlling this process is unknown but does not involve any change in the host cell type or parasitophorous vacuole and may be pre-programmed, since the number of asexual cycles was self-limiting. In conclusion, it was possible using a combination of molecular markers to identify tachyzoite, bradyzoite and coccidian development in tissue sections.

PubMed: 15003495, full text

Localisation information

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: parasite plasma membrane during tachyzoite, not bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Tachyzoites showing brown surface labelling with anti-SAG1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: surface during tachyzoite, not during bradyzoite

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: not merozoite, not sporozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, not during sporozoite

TGME49_033480 (SRS2) SRS29C (= SRS2, P35)

Experimental localisation: tachyzoite, not bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: during tachyzoite, not during bradyzoite

TGME49_033480 (SRS2) SRS29C (= SRS2, P35)

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_007160 (SAG2C) SRS49D (= SAG2C)

Experimental localisation: tachyzoite, not bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: during tachyzoite, not during bradyzoite

TGME49_007160 (SAG2C) SRS49D (= SAG2C)

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: not tachyzoite, parasite plasma membrane during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Of the surface markers, only BSR4 showed any labelling."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: blast from AF123660, annotation matches
  • Localisation record: not during tachyzoite, surface during bradyzoite

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: parasite plasma membrane during merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Of the surface markers, only BSR4 showed any labelling."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: Surface during merozoite

TGME49_059020 (BAG1) bradyzoite antigen, putative

Experimental localisation: not tachyzoite, cytoplasm during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 (e) Tissue cyst showing labelling of the cyst wall with Ab CC2 (red) and cytoplasmic labelling of the bradyzoites with anti-BAG1 (green)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during tachyzoite, cytoplasm during bradyzoite

TGME49_059020 (BAG1) bradyzoite antigen, putative

Experimental localisation: not merozoite, not sporozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, not during sporozoite

TGME49_068860 (ENO1) enolase 1

Experimental localisation: not tachyzoite, bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during tachyzoite, during bradyzoite

TGME49_068860 (ENO1) enolase 1

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_068850 (ENO2) enolase 2

Experimental localisation: cytoplasm during tachyzoite, nucleus during tachyzoite, not bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 (d) A group of tachyzoites showing surface labelling with anti-SAG1 (green) and cytoplasmic and particularly nuclear labelling with anti-ENO2 (red)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: cytoplasm and nuclear during tachyzoite, not during bradyzoite

TGME49_068850 (ENO2) enolase 2

Experimental localisation: cytoplasm during merozoite, nucleus during merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "(f) Coccidian stages showing cytoplasmic labelling with anti-LHD1 (red) and ENO2 (green). Note the very strong labelling of the nuclei with anti-ENO2."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: Cytoplasm and nucleus during merozoite

TGME49_032350 (lactate dehydrogenase-1, LDH1) lactate dehydrogenase

Experimental localisation: tachyzoite, not bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: during tachyzoite, not during bradyzoite

TGME49_032350 (lactate dehydrogenase-1, LDH1) lactate dehydrogenase

Experimental localisation: cytoplasm during merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "(f) Coccidian stages showing cytoplasmic labelling with anti-LHD1 (red) and ENO2 (green)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: cytoplasm during merozoite

TGME49_091040 (LDH2) lactate dehydrogenase

Experimental localisation: not tachyzoite, bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during tachyzoite, during bradyzoite

TGME49_091040 (LDH2) lactate dehydrogenase

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_070250 (GRA1) dense granule protein 1 / major antigenp24

Experimental localisation: parasitophorous vacuole during tachyzoite, parasitophorous vacuole during bradyzoite, oocyst wall during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite and bradyzoite, cyst wall during bradyzoite

TGME49_070250 (GRA1) dense granule protein 1 / major antigenp24

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_027620 (GRA2) 28 kDa antigen

Experimental localisation: parasitophorous vacuole during tachyzoite, parasitophorous vacuole during bradyzoite, not oocyst wall during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite and bradyzoite, not cyst wall during bradyzoite

TGME49_027620 (GRA2) 28 kDa antigen

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_027280 (GRA3) dense granule protein 3

Experimental localisation: parasitophorous vacuole during tachyzoite, parasitophorous vacuole during bradyzoite, oocyst wall during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite and bradyzoite, cyst wall during bradyzoite

TGME49_027280 (GRA3) dense granule protein 3

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_110780 (GRA4) hypothetical protein

Experimental localisation: parasitophorous vacuole during tachyzoite, bradyzoite, not parasitophorous vacuole during bradyzoite, not oocyst wall during bradyzoite, not parasite plasma membrane during bradyzoite, dense granule during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j). In the case of the tissue cyst, it was observed that for GRA4, while adjacent dense granules within bradyzoites were labelled, the cyst wall was negative (Fig. 7e). (e) Part of a tissue cyst labelled with anti-GRA4 showing a number of 5 nm gold particles associated with a dense granule within a bradyzoite but no labelling of the cyst wall (CW) or limiting membrane (arrowheads)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite, during bradyzoite, not PV during bradyzoite, not cyst wall and not limiting membrane and dense granule during bradyzoite

TGME49_110780 (GRA4) hypothetical protein

Experimental localisation: not merozoite, sporozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, during sporozoite

TGME49_086450 (GRA5) dense granule protein 5 precursor

Experimental localisation: parasitophorous vacuole during tachyzoite, parasitophorous vacuole during bradyzoite, oocyst wall during bradyzoite, parasitophorous vacuole membrane during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j). When stained for GRA5 or 6, it was observed that the gold particles were specifically associated with the limiting membrane of the PV (Fig. 7f and g)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite and bradyzoite, cyst wall and limiting membrane of PV during bradyzoite

TGME49_086450 (GRA5) dense granule protein 5 precursor

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_075440 (GRA6) granule antigen protein GRA6

Experimental localisation: tubular structure in parasitophorous vacuole during tachyzoite, tubular structure in parasitophorous vacuole during bradyzoite, dense granule during tachyzoite, dense granule during bradyzoite, outer membrane of cyst wall during bradyzoite, parasitophorous vacuole membrane during bradyzoite, not dense granule during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j). Note the absence of staining of the dense granules (DG) within the bradyzoites, which were strongly labelled with anti-GRA6 (green). By immuno-electron microscopy, it was observed, during tachyzoite development, that the dense granule proteins strongly labelled structures within the PV. For example, there was strong labelling of the tubular structure for GRA6 (Fig. 7c). When stained for GRA5 or 6, it was observed that the gold particles were specifically associated with the limiting membrane of the PV (Fig. 7f and g). This difference in distribution within the cyst wall could be seen in sections double labelled with anti-GRA6 and CC2, where the GRA6 was located on the outer membrane and CC2 throughout the wall (Fig. 7g)."
  • Microscopy type: Light, EM
  • Microscopy method: Monoclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: Tubular structures in PV and dense granule during tachyzoite and bradyzoite, outer membrane of cyst wall and limiting membrane of PV and not dense granule during bradyzoite

TGME49_075440 (GRA6) granule antigen protein GRA6

Experimental localisation: not merozoite, sporozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: Monoclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, during sporozoite

TGME49_003310 (GRA7) dense granule protein 7

Experimental localisation: parasitophorous vacuole during tachyzoite, parasitophorous vacuole during bradyzoite, oocyst wall during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 (c) Early stages of coccidian development showing the parasitophorous vacuoles labelled with anti-GRA7 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite and bradyzoite, cyst wall during bradyzoite

TGME49_003310 (GRA7) dense granule protein 7

Experimental localisation: parasitophorous vacuole during sporozoite, parasitophorous vacuole during early merozoite, not parasitophorous vacuole during mature schizont merozoite, dense granule during mature schizont merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 The GRA7 staining of the PV was strong during early development (Fig. 3c) and was reduced in the later stages where strong staining was limited to the dense granules in the apical cytoplasm of the merozoites in mature schizonts (Fig. 9c). Using double labelling, it was possible to show that NTPase was located in granules separated from the rhoptries (Fig. 9f) and that the GRA7 and NTPase were co-localised in these granules (Fig. 9g)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during sporozoite, PV during early merozoite, not PV and dense granule during mature merozoite schizont

TGME49_054720 (GRA8) dense granule protein GRA8

Experimental localisation: parasitophorous vacuole during tachyzoite, not parasitophorous vacuole during bradyzoite, not oocyst wall during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite, not PV during bradyzoite, not cyst wall during bradyzoite

TGME49_054720 (GRA8) dense granule protein GRA8

Experimental localisation: not merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite

TGME49_077240 (NTPase, NTP1) nucleoside-triphosphatase I

Experimental localisation: parasitophorous vacuole during tachyzoite, oocyst wall during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 In contrast, the level of labelling of the cyst wall was very variable (Fig. 5b). There was positive staining with GRA1 (Fig. 5b) GRA3 (Fig. 5d), GRA5 (Fig. 5f), GRA6 (Fig. 5g) and GRA7 (Fig. 5h) and reduced staining with GRA2 (Fig. 5c), GRA4 (Fig. 5e), GRA8 (Fig. 5i) and NTPase (Fig. 5j)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RRA
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV during tachyzoite, weak cyst wall during bradyzoite

TGME49_077240 (NTPase, NTP1) nucleoside-triphosphatase I

Experimental localisation: parasitophorous vacuole during merozoite, dense granule during merozoite, not rhoptry during merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Table 1 Table 2 Using double labelling, it was possible to show that NTPase was located in granules separated from the rhoptries (Fig. 9f) and that the GRA7 and NTPase were co-localised in these granules (Fig. 9g)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: PV and dense granule and not rhoptry during merozoite

TGME49_015780 (ROP2/3/4, ROP2, Tg3, Tg4, Tg34) Rhoptry kinase family protein ROP2A (incomplete catalytic triad)

Experimental localisation: rhoptry during mature schizont merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Section through a mature schizont double labelled with anti-ROP2-4 (red) and anti-NTPase (green) showing the rhoptry protein was in separate granules from the dense granule protein (from Ferguson et al., 1999a)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: rhoptry during mature schizont merozoite