ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Subcellular localization of 14-3-3 proteins in Toxoplasma gondii tachyzoites and evidence for a lipid raft-associated form.

Assossou, O., Besson, F., Rouault, J. P., Persat, F., Brisson, C., Duret, L., Ferrandiz, J., Mayencon, M., Peyron, F., Picot, S. (2003 Jul 29, FEMS Microbiol Lett)

A polyclonal antibody was raised against a Toxoplasma gondii 14-3-3-gluthatione S-transferase fusion protein obtained by cloning a 14-3-3 cDNA sequence determined from the T. gondii database. This antibody specifically recognized T. gondii 14-3-3 without any cross-reaction with mammalian proteins. Immunofluorescence microscopy studies of the tachyzoites or the T. gondii-infected cells suggested cytosolic and membranous localizations of 14-3-3 protein. Different subcellular fractions were prepared for electrophoresis analysis and immunodetection. 14-3-3 proteins were found in the cytosol, the membrane fraction and Triton X-100-resistant membranes. Two 14-3-3 isoforms were detected. The major one was mainly cytoplasmic and to a lesser extent membrane-associated, whereas the minor isoform was associated with the detergent-resistant lipid rafts.

PubMed: 12892878, full text

Localisation information

TGME49_063090 14-3-3 protein, putative

Experimental localisation: cytosol during extracellular tachyzoite, parasite plasma membrane during extracellular tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Detection by immunofluorescence microscopy showed that 14-3-3 protein is cytosolic since it could be detected in permeabilized parasites, along with the cytosolic GRA1 protein. 14-3-3 protein is also membrane-associated, as evidenced by its colocalization with SAG1 protein in non-permeabilized parasites."
  • Microscopy type: Light
  • Microscopy method: antibody directly to protein
  • Strain: RH
  • Gene model mapping comments: Blast from NCU31542, annotation matches
  • Localisation record: cytosol and membrane during free tachyzoite
  • Comment: Called 14-3-3, but openoffice calc seems to convert it to date format all the time, messing this up