ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Aldolase forms a bridge between cell surface adhesins and the actin cytoskeleton in apicomplexan parasites.

Jewett, T. J., Sibley, L. D. (2003 Apr, Mol Cell)

Host cell invasion by apicomplexan parasites requires coordinated interactions between cell surface adhesins and the parasite cytoskeleton. We have identified a complex of parasite proteins, including the actin binding protein aldolase, which specifically interacts with the C-terminal domains of several parasite adhesins belonging to the thrombospondin-related anonymous protein (TRAP) family. Binding of aldolase to the adhesin was disrupted by mutation of a critical tryptophan in the C domain, a residue that was previously shown to be essential for parasite motility. Our findings reveal a potential role for aldolase in connecting TRAP family adhesins with the cytoskeleton, and provide a model linking adhesion with motility in apicomplexan parasites.

PubMed: 12718875, full text

Localisation information

TGME49_036040 (Aldolase I, aldolase, aldolase-1) fructose-1,6-bisphosphate aldolase

Experimental localisation: apical during tachyzoite, conoid during tachyzoite gliding motility
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Toxoplasma gondii Aldolase Is Apically Localized and Partially Relocalized during Gliding Motility...In gliding parasites where MIC2 had translocated toward the posterior end of the cell, a portion of the cellular aldolase was also redistributed from its normal apical location"
  • Microscopy type: Light
  • Microscopy method: antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from AY150663
  • Localisation record: apical during tachyzoite, conoid during tachyzoite gliding motility
  • Comment: The Toxoplasma genome contains two potential aldolase-encoding sequences, but only one of these, aldolase-1, is expressed in Toxoplasma tachyzoites and the second appears to represent a pseudogene ([7], data not shown).