ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Male-specific expression of the paralog of malaria transmission-blocking target antigen Pfs230, PfB0400w.

Eksi, S., Williamson, K. C. (2002 Jul, Mol Biochem Parasitol)

Malaria transmission requires that Plasmodium parasites circulating in the vertebrate host develop into male and female gametocytes, which are then taken up by a mosquito to undergo fertilization and further development into infectious sporozoites. To understand the malaria specific events involved in this process, the gene products involved require identification and characterization. This work demonstrates that antibodies generated against the paralog of malaria transmission-blocking antigen Pfs230, PfB0400w, react only with stage V male gametocytes, not gametes or asexual parasites. In contrast, Pfs230 is expressed on the surface of all gametocytes and remains associated with emerged gametes as one of the primary surface antigens for several hours. Consistent with the localization findings, a high molecular weight band is recognized by anti-PfB0400w antibodies on western blots of extracts of late stage gametocytes, not asexual parasites, early (stage II/III) gametocytes, or gametes. PfB0400w mRNA is also not observed in asexual parasites. The transcript levels peak in stage III/IV gametocytes, then sharply decline in gametes. This work identifies a novel male-specific protein with an expression pattern that is distinctly different than its paralog.

PubMed: 12106866, full text

Localisation information

PFD1050w (alpha tubulin II, a-tubulin II) alpha tubulin 2

Experimental localisation: male gametocyte stage v
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "The stage V gametocytes that were positive for PfB0400w also stained with anti--tubulin II antibodies and exhibited diffuse nuclear staining with 4,6-diamidino-2-phenylindole (DAPI), suggesting that they were male gametocytes (Fig. 2A)"
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody against homologue
  • Strain:
  • Gene model mapping comments: inferred from another publication
  • Localisation record: during stage V male gametocyte
  • Comment: localisation not annunciated
  • Comment: strain information not found

PFB0400w 6-cysteine protein

Experimental localisation: cytoplasm during gametocyte stage v, not emerged gametocyte
  • Species: Plasmodium falciparum
  • Quote inferring localisation: "Only a small fraction of the stage V gametocytes were recognized and the fluorescence pattern was diffuse and patchy, which is not consistent with the rim fluorescence pattern observed with surface antigens, such as Pfs230. The fluorescence pattern is more consistent with localized concentrations of the antigen in the cytoplasm. This pattern coupled with PfB0400w's secretory signal sequence suggests that it may be located in cytoplasmic vesicles. The stage V gametocytes that were positive for PfB0400w also stained with anti--tubulin II antibodies and exhibited diffuse nuclear staining with 4,6-diamidino-2-phenylindole (DAPI), suggesting that they were male gametocytes (Fig. 2A) [15. "
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: inferred from another publication
  • Localisation record: cytoplasm during stage V gametocyte, not during after gametocyte emergence
  • Comment: strain information not found