ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Toxoplasma gondii: molecular cloning and characterization of a novel 18-kDa secretory antigen, TgMIC10.

Hoff, E. F., Cook, S. H., Sherman, G. D., Harper, J. M., Ferguson, D. J., Dubremetz, J. F., Carruthers, V. B. (2001 Feb, Exp Parasitol)

Hoff, E. F., Cook, S. H., Sherman, G. D., Harper, J. M., Ferguson, D. J. P., Dubremetz, J. F., and Carruthers, V. B. 2001. Toxoplasma gondii: Molecular cloning and characterization of a novel 18-kDa secretory antigen, TgMIC10. Experimental Parasitology, 97, 77-88. During host cell invasion, Toxoplasma gondii secretes proteins from specialized organelles (micronemes and rhoptries) located at the apical end of the parasite. The contents of the micronemes appear to be crucial to T. gondii invasion, as inhibition of microneme secretion prevents parasite entry into host cells. Here we describe a new T. gondii microneme protein, TgMIC10. Molecular characterization of a full-length TgMIC10 cDNA revealed that TgMIC10 lacks homology to any previously characterized proteins, although a homologue, NcMIC10, was identified in a closely related parasite, Neospora caninum. TgMIC10 has an unusually long secretory leader sequence of 58 amino acids; the mature TgMIC10 is 18 kDa, possesses nine diglutamic acid repeats and an imperfect repeat sequence (RK(R/Y)HEEL), and is entirely devoid of cysteines. Antibodies raised against recombinant TgMIC10 recognized the native TgMIC10 and localized the protein to the micronemes in indirect immunofluorescence and immunoEM experiments. Comparison of immunofluorescence images indicates that TgMIC10 expression is higher in T. gondii tachyzoites, which are responsible for active infection, than in bradyzoites, which are responsible for latent infection.

PubMed: 11281704, full text

Localisation information

TGME49_033460 (SAG1, SRS29B, P30/SAG1, BSR4, P30) SRS29B (= SAG1, P30)

Experimental localisation: parasite plasma membrane
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TgSAG1 (a uniformly distributed T. gondii surface protein)"
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH, 2F
  • Gene model mapping comments: inferred from another publication
  • Localisation record: Surface

TGME49_015780 (ROP2/3/4, ROP2, Tg3, Tg4, Tg34) Rhoptry kinase family protein ROP2A (incomplete catalytic triad)

Experimental localisation: rhoptry
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "(three antigenically cross-reacting rhoptry proteins)"
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: RH, 2F
  • Gene model mapping comments: inferred from another publication
  • Localisation record: rhoptry

TGME49_008030 (MIC4, MIC 4) microneme protein MIC4

Experimental localisation: microneme
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "(another micronemal protein)"
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain: RH, 2F
  • Gene model mapping comments: inferred from another publication
  • Localisation record: microneme

TGME49_050710 (MIC10) microneme protein 10

Experimental localisation: microneme during tachyzoite, bradyzoite, microneme during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Section of brain showing a group of tachyzoites immunostained with anti-rTgMIC10 (red) and anti-TgSAG1 (green). Bar, 5 um. (B) Brain section immunostained with anti-rTgMIC10 (green) and anti-TgROP2,3,4 (red), showing the separate location of the two targets within the apical region of the tachyzoites."
  • Microscopy type: Light, cryo-immunoEM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH, 2F
  • Gene model mapping comments: Blast from AF293654, annotation matches
  • Localisation record: microneme during tachyzoite, reduced and microneme during bradyzoite