ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

Subcellular localization of acetyl-CoA carboxylase in the apicomplexan parasite Toxoplasma gondii.

Jelenska, J., Crawford, M. J., Harb, O. S., Zuther, E., Haselkorn, R., Roos, D. S., Gornicki, P. (2001 Feb 27, Proc Natl Acad Sci U S A)

Apicomplexan parasites such as Toxoplasma gondii contain a primitive plastid, the apicoplast, whose genome consists of a 35-kb circular DNA related to the plastid DNA of plants. Plants synthesize fatty acids in their plastids. The first committed step in fatty acid synthesis is catalyzed by acetyl-CoA carboxylase (ACC). This enzyme is encoded in the nucleus, synthesized in the cytosol, and transported into the plastid. In the present work, two genes encoding ACC from T. gondii were cloned and the gene structure was determined. Both ORFs encode multidomain proteins, each with an N-terminal extension, compared with the cytosolic ACCs from plants. The N-terminal extension of one isozyme, ACC1, was shown to target green fluorescent protein to the apicoplast of T. gondii. In addition, the apicoplast contains a biotinylated protein, consistent with the assertion that ACC1 is localized there. The second ACC in T. gondii appears to be cytosolic. T. gondii mitochondria also contain a biotinylated protein, probably pyruvate carboxylase. These results confirm the essential nature of the apicoplast and explain the inhibition of parasite growth in cultured cells by herbicides targeting ACC.

PubMed: 11226307, full text

Localisation information

TGME49_021320 (ACC1) acetyl-CoA carboxylase, putative

Experimental localisation: apicoplast
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "The N-terminal extension of one isozyme, ACC1, was shown to target green fluorescent protein to the apicoplast of T. gondii."
  • Microscopy type: Light
  • Microscopy method: GFP tag
  • Strain: RH
  • Gene model mapping comments: blast from AF157612, annotation matches
  • Localisation record: apicoplast
  • Comment: gene model inconsistent

TGME49_018560 (ACC2) acetyl-coA carboxylase

Experimental localisation: cytosol
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "The second ACC in T. gondii appears to be cytosolic."
  • Microscopy type: Light
  • Microscopy method: GFP tag
  • Strain: RH
  • Gene model mapping comments: blast from AF157613, annotation matches
  • Localisation record: cytosol
  • Comment: gene model inconsistent Alternate splicing?