ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

A plasma membrane-type Ca(2+)-ATPase co-localizes with a vacuolar H(+)-pyrophosphatase to acidocalcisomes of Toxoplasma gondii.

Luo, S., Vieira, M., Graves, J., Zhong, L., Moreno, S. N. (2001 Jan 15, EMBO J)

Ca(2+)-ATPases are likely to play critical roles in the biochemistry of Toxoplasma gondii, since these protozoa are obligate intracellular parasites and the Ca(2+) concentration in their intracellular location is three orders of magnitude lower than in the extracellular medium. Here, we report the cloning and sequencing of a gene encoding a plasma membrane-type Ca(2+)-ATPase (PMCA) of T.gondii (TgA1). The predicted protein (TgA1) exhibits 32-36% identity to vacuolar Ca(2+)-ATPases of Trypanosoma cruzi, Saccharomyces cerevisiae, Entamoeba histolytica and Dictyostelium discoideum. Sequencing of both cDNA and genomic DNA from T.gondii indicated that TgA1 contains two introns near the C-terminus. A hydropathy profile of the protein suggests 10 transmembrane domains. TgA1 suppresses the Ca(2+) hypersensitivity of a mutant of S.cerevisiae that has a defect in vacuolar Ca(2+) accumulation. Indirect immunofluorescence and immunoelectron microscopy analysis indicate that TgA1 localizes to the plasma membrane and co-localizes with the vacuolar H(+)-pyrophosphatase to intracellular vacuoles identified morphologically and by X-ray microanalysis as the acidocalcisomes. This vacuolar-type Ca(2+)-ATPase could play an important role in Ca(2+) homeostasis in T.gondii.

PubMed: 11226155, full text

Localisation information

TGME49_112100 (A1, PMCA) Ca2+-ATPase

Experimental localisation: acidocalcisome during tachyzoite, acidocalcisome during bradyzoite, parasite plasma membrane during tachyzoite, parasite plasma membrane during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Indirect immunofluorescence and immunoelectron microscopy analysis indicate that TgA1 localizes to the plasma membrane and co-localizes with the vacuolar H+-pyrophosphatase to intracellular vacuoles identified morphologically and by X-ray microanalysis as the acidocalcisomes."
  • Microscopy type: EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH
  • Gene model mapping comments: Blast from AF151371
  • Localisation record: strong acidocalcisomes and weak cell surface during tachyzoite and bradyzoite

TGME49_112100 (A1, PMCA) Ca2+-ATPase

Experimental localisation: acidocalcisome during tachyzoite, acidocalcisome during bradyzoite, parasite plasma membrane during tachyzoite, parasite plasma membrane during bradyzoite, not dense granule during tachyzoite, not dense granule during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Indirect immunofluorescence and immunoelectron microscopy analysis indicate that TgA1 localizes to the plasma membrane and co-localizes with the vacuolar H+-pyrophosphatase to intracellular vacuoles identified morphologically and by X-ray microanalysis as the acidocalcisomes."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: ME49
  • Gene model mapping comments: inferred from another publication
  • Localisation record: strong acidocalcisomes and weak cell surface and not dense granule during tachyzoite and bradyzoite

TGME49_027620 (GRA2) 28 kDa antigen

Experimental localisation: dense granule during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "Co-localization studies with the antibody against the V-H+-PPase and an antibody against the dense granule marker GRA2 (Labruyere et al., 1999) were also performed to rule out that the organelles identifed as acidocalcisomes were the dense granules."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: ME49
  • Gene model mapping comments: inferred from another publication
  • Localisation record: dense granule during tachyzoite

TGME49_048670 (VP1, V-H+-PPase, VP-1) H+-translocating inorganic pyrophosphatase TVP, putative

Experimental localisation: acidocalcisome during tachyzoite, not dense granule during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "We also report, for the first time, the localization of the V-H+-PPase to the acidocalcisomes of T.gondii."
  • Microscopy type: EM
  • Microscopy method: polyclonal antibody to homologue
  • Strain: RH
  • Gene model mapping comments: Blast from epitope CNTGGAWDNAKKYIEKGGLRDKKGKGS, annotation matches
  • Localisation record: acidocalcisomes and not dense granule during tachyzoite