ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

The expression and distribution of dense granule proteins in the enteric (Coccidian) forms of Toxoplasma gondii in the small intestine of the cat.

Ferguson, D. J., Cesbron-Delauw, M. F., Dubremetz, J. F., Sibley, L. D., Joiner, K. A., Wright, S. (1999 Mar, Exp Parasitol)

The expression and distribution of dense granule proteins in the enteric (coccidian) forms of Toxoplasma gondii in the small intestine of the cat. Experimental Parasitology 91, 203-211. The expression and location of the dense granule proteins (GRA1-6 and NTPase) in the merozoite and during asexual and sexual development of Toxoplasma gondii in the small intestine of the cat (definitive host) was examined by immuno-light and electron microscopy. This was compared with that of tachyzoites and bradyzoites present in the intermediate host. It was found that the merozoite contained the characteristic apical organelles plus a few large dense granules. By immunocytochemistry, dense granules in merozoites were negative for GRA proteins 1 to 6 in contrast to both tachyzoites and bradyzoites in which dense granules were positive for all six proteins. The GRA proteins were associated with the parasitophorous vacuole (PV) during tachyzoite and bradyzoite development but were absent from the PV of the enteric stages. However, the merozoite dense granules were positive for NTPase, which was similar to the tachyzoite while this antigen was down regulated in the bradyzoite. The apparent release of the NTPases into the PV formed by merozoites was also similar to that described for the tachyzoite, possibly reflecting the relative metabolic activity of the various stages. This study shows that the majority of GRA proteins have a similar stage-specific expression, which is independent of NTPases expression. These observations are consistent with T. gondii having a different host parasite relationship in the enteric forms, which does not involve the GRA proteins 1-6.

PubMed: 10072322, full text

Localisation information

TGME49_070250 (GRA1) dense granule protein 1 / major antigenp24

Experimental localisation: not merozoite, dense granule during tachyzoite, dense granule during bradyzoite, dense granule during sporozoite, parasitophorous vacuole during tachyzoite, oocyst wall during tachyzoite, not parasitophorous vacuole during merozoite, not parasitophorous vacuole during sporozoite, not parasitophorous vacuole during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, dense granule during tachyzoite and bradyzoite and sporozoite, PV and cyst wall during tachyzoite, not PV during merozoite and sporozoite and bradyzoite

TGME49_027620 (GRA2) 28 kDa antigen

Experimental localisation: not merozoite, not bradyzoite, not sporozoite, dense granule during tachyzoite, parasitophorous vacuole during tachyzoite, oocyst wall during tachyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite and bradyzoite and sporozoite, dense granule and PV and cyst wall during tachyzoite

TGME49_027280 (GRA3) dense granule protein 3

Experimental localisation: not merozoite, not sporozoite, not bradyzoite, dense granule during tachyzoite, parasitophorous vacuole during tachyzoite, oocyst wall during tachyzoite, not parasitophorous vacuole during merozoite, not parasitophorous vacuole during sporozoite, not parasitophorous vacuole during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite and sporozoite and bradyzoite, dense granule during tachyzoite, PV and cyst wall during tachyzoite, not PV during merozoite and sporozoite and bradyzoite

TGME49_110780 (GRA4) hypothetical protein

Experimental localisation: not merozoite, dense granule during tachyzoite, dense granule during bradyzoite, dense granule during sporozoite, parasitophorous vacuole during tachyzoite, oocyst wall during tachyzoite, not parasitophorous vacuole during merozoite, not parasitophorous vacuole during sporozoite, not parasitophorous vacuole during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: Monoclonal antibody directly to protein, polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, dense granule during tachyzoite and bradyzoite and sporozoite, PV and cyst wall and cyst wall during tachyzoite, not PV during merozoite and sporozoite and bradyzoite

TGME49_086450 (GRA5) dense granule protein 5 precursor

Experimental localisation: not merozoite, dense granule during tachyzoite, parasitophorous vacuole during tachyzoite, oocyst wall during tachyzoite, dense granule during bradyzoite, dense granule during sporozoite, not parasitophorous vacuole during bradyzoite, not parasitophorous vacuole during sporozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, dense granule and PV and cyst wall during tachyzoite, dense granule and not PV during bradyzoite and sporozoite

TGME49_075440 (GRA6) granule antigen protein GRA6

Experimental localisation: not merozoite, dense granule during tachyzoite, dense granule during bradyzoite, dense granule during sporozoite, parasitophorous vacuole during tachyzoite, oocyst wall during tachyzoite, not parasitophorous vacuole during merozoite, not parasitophorous vacuole during sporozoite, not parasitophorous vacuole during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: Monoclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: not during merozoite, dense granule during tachyzoite and bradyzoite and sporozoite, PV and cyst wall during tachyzoite, not PV during merozoite and sporozoite and bradyzoite

TGME49_077240 (NTPase, NTP1) nucleoside-triphosphatase I

Experimental localisation: dense granule during merozoite, dense granule during tachyzoite, parasitophorous vacuole during merozoite, parasitophorous vacuole during tachyzoite, not rhoptry during merozoite, not rhoptry during tachyzoite, not sporozoite, parasitophorous vacuole during bradyzoite, dense granule during bradyzoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "TABLE I The Expression of Dense Granule Proteins in the Various Infectious Forms of T. gondii"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: dense granule and PV and not rhoptry during merozoite and tachyzoite, not during sporozoite, little PV and dense granule during bradyzoite

TGME49_015780 (ROP2/3/4, ROP2, Tg3, Tg4, Tg34) Rhoptry kinase family protein ROP2A (incomplete catalytic triad)

Experimental localisation: rhoptry during merozoite
  • Species: Toxoplasma gondii
  • Quote inferring localisation: "However, by double labelling using anti-ROP2,3,4 and anti-NTPases it was shown that NTPases are located in a distinct structure located between the nucleus and the rhoptries (Fig. 1h)."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: M3
  • Gene model mapping comments: inferred from another publication
  • Localisation record: rhoptries during merozoite