ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

P. falciparum Maurer's Cleft Vincensini et al 2005

Proteomic analysis identifies novel proteins of the Maurer's clefts, a secretory compartment delivering Plasmodium falciparum proteins to the surface of its host cell.

Vincensini, L., Richert, S., Blisnick, T., Van Dorsselaer, A., Leize-Wagner, E., Rabilloud, T., Braun Breton, C. (2005 Apr)

A novel method was validated for the efficient distinction between malaria parasite-derived and host cell proteins in mass spectrometry analyses. This method was applied to a ghost fraction from Plasmodium falciparum-infected erythrocytes containing the red blood cell plasma membrane, the erythrocyte submembrane skeleton, and the Maurer's clefts, a Golgi-like apparatus linked to and addressing parasite proteins to the host cell surface. This method allowed the identification of 78 parasite proteins. Among these we identified seven novel proteins of the Maurer's clefts based on immunofluorescence studies and proteinase K digestion assays. The products of six contiguous genes located on chromosome 5 were identified, and the location within the Maurer's clefts was established for two of them. This suggests a clustering of genes encoding Maurer's cleft proteins. Our study sheds new light on the biological function of the Maurer's clefts, which are central to the pathogenesis and to the intraerythrocytic development of P. falciparum.

PubMed: 15671043, full text

Proteins Found:

  • MAL13P1.214 (pmt) phosphoethanolamine N-methyltransferase
  • MAL7P1.174 Plasmodium exported protein (PHISTb), unknown function
  • MAL8P1.17 (Pf52, PDI, DSI) protein disulfide isomerase
  • MAL8P1.69 14-3-3 protein, putative
  • PF07_0029 heat shock protein 90
  • PF08_0019 receptor for activated c kinase
  • PF08_0054 (hsp, Hsp70-1) heat shock protein 70
  • PF10_0013 Plasmodium exported protein (hyp12), unknown function
  • PF10_0023 (J23) Plasmodium exported protein (hyp16), unknown function
  • PF10_0086 adenylate kinase
  • PF10_0121 (hypoxanthine phosphoribosyl transferase) hypoxanthine phosphoribosyltransferase
  • PF10_0155 (enolase) enolase
  • PF10_0159 (GBP130) glycophorin binding protein
  • PF10_0323 (ETRAMP10.2, J323) early transcribed membrane protein 10.2
  • PF10_0366 ADP/ATP transporter on adenylate translocase
  • PF11_0055 conserved protein, unknown function
  • PF11_0183 GTP-binding nuclear protein ran/tc4
  • PF11_0224 (exp-1, EXP1, EXP-1) circumsporozoite-related antigen
  • PF11_0351 heat shock protein 70
  • PF13_0141 L-lactate dehydrogenase
  • PF13_0197 (p22, MSP7) merozoite surface protein 7 precursor
  • PF14_0378 triosephosphate isomerase
  • PF14_0425 (aldolase, pfAldolase) fructose-bisphosphate aldolase
  • PF14_0434 conserved Plasmodium protein, unknown function
  • PF14_0541 (VP1, V-type H(+)-translocating pyrophosphatase) V-type H( )-translocating pyrophosphatase, putative
  • PF14_0598 (pfGAPDH) glyceraldehyde-3-phosphate dehydrogenase
  • PF14_0678 (EXP2, EXP-2) exported protein 2
  • PFA0680c (A680) Pfmc-2TM Maurer's cleft two transmembrane protein
  • PFB0100c (KAHRP, HRP1) knob-associated histidine-rich protein
  • PFB0335c (SERP H, SERA6, SERP-H) serine repeat antigen 6
  • PFB0340c (SERA5, SERP, p126, SERA, pl26) serine repeat antigen 5
  • PFD0080c Plasmodium exported protein (PHISTb), unknown function
  • PFD0310w (Pfs16) sexual stage-specific protein precursor
  • PFD1170c Plasmodium exported protein (PHISTb), unknown function
  • PFE0050w Plasmodium exported protein, unknown function
  • PFE0055c (PFE55) heat shock protein 40, type II
  • PFE0060w (E60) parasite-infected erythrocyte surface protein
  • PFE0065w (SBP1, SBP) skeleton-binding protein 1
  • PFE0075c rhoptry-associated protein 3
  • PFE0660c purine nucleoside phosphorylase
  • PFE0760w conserved Plasmodium protein, unknown function
  • PFE1600w Plasmodium exported protein (PHISTb), unknown function
  • PFF1300w pyruvate kinase
  • PFI0875w (grp, Hsp70-2, BiP) heat shock protein 70
  • PFI1445w (RhopH2) high molecular weight rhoptry protein 2
  • PFI1475w (MSP1, MSP-1, msp1 unprocessed, msp1, Msp-1 19, MSP1 19) merozoite surface protein 1
  • PFL0050c Plasmodium exported protein (PHISTb), unknown function
  • PFL0780w glycerol-3-phosphate dehydrogenase, putative
  • PFL1070c endoplasmin homolog precursor, putative
  • PFL1385c (ABRA, MSP9, MSP-9) merozoite surface protein 9