ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

P. falciparum Food Vacuole Lamarque et al 2008

Food vacuole proteome of the malarial parasite Plasmodium falciparum

Mauld Lamarque, Christophe Tastet, Joël Poncet, Edith Demettre, Patrick Jouin, Henri Vial, Jean-François Dubremetz, Dr. (2008)

The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P. falciparum.

http://www3.interscience.wiley.com/journal/120840762/abstract

Proteins Found:

  • MAL13P1.214 (pmt) phosphoethanolamine N-methyltransferase
  • MAL13P1.221 aspartate carbamoyltransferase
  • MAL13P1.231 (Sec61) Sec61 alpha subunit, PfSec61
  • MAL13P1.233 DNA/RNA-binding protein Alba, putative
  • MAL13P1.237 conserved Plasmodium protein, unknown function
  • MAL13P1.289 mitotic control protein dis3 homologue, putative
  • MAL13P1.56 (A-M1) M1-family alanyl aminopeptidase
  • MAL7P1.27 (CRT) chloroquine resistance transporter
  • MAL7P1.67 conserved Plasmodium protein, unknown function
  • MAL8P1.17 (Pf52, PDI, DSI) protein disulfide isomerase
  • MAL8P1.69 14-3-3 protein, putative
  • MAL8P1.95 conserved Plasmodium protein, unknown function
  • PF07_0029 heat shock protein 90
  • PF08_0032 DnaJ protein, putative
  • PF08_0054 (hsp, Hsp70-1) heat shock protein 70
  • PF08_0064 vacuolar protein-sorting protein VPS2, putative
  • PF08_0074 DNA/RNA-binding protein Alba, putative
  • PF08_0091 conserved Plasmodium protein, unknown function
  • PF10_0028-b RNA binding protein, putative
  • PF10_0063 DNA/RNA-binding protein Alba, putative
  • PF10_0068 RNA binding protein, putative
  • PF10_0084 tubulin beta chain
  • PF10_0126 conserved Plasmodium protein, unknown function
  • PF10_0130 conserved Plasmodium protein, unknown function
  • PF10_0153 (Cpn60, Hsp60, hsp60) heat shock protein 60
  • PF10_0159 (GBP130) glycophorin binding protein
  • PF10_0203 ADP-ribosylation factor
  • PF10_0351 probable protein, unknown function
  • PF10_0366 ADP/ATP transporter on adenylate translocase
  • PF10_0372-b antigen UB05
  • PF11_0055 conserved protein, unknown function
  • PF11_0069 conserved Plasmodium protein, unknown function
  • PF11_0098 (ERC, ERC1, Pfs40, Pf39) endoplasmic reticulum-resident calcium binding protein
  • PF11_0165 (Falcipain-2, FP2) cysteine proteinase falcipain 2a
  • PF11_0168 (RON4, Pf225) rhoptry neck protein 4
  • PF11_0175 (HSP101, ClpB2) heat shock protein 101
  • PF11_0183 GTP-binding nuclear protein ran/tc4
  • PF11_0224 (exp-1, EXP1, EXP-1) circumsporozoite-related antigen
  • PF11_0281 protein phosphatase, putative
  • PF11_0301 (SPDS) spermidine synthase
  • PF11_0302 (PV-1) parasitophorous vacuolar protein 1
  • PF11_0338 (AQP) aquaglyceroporin
  • PF11_0351 heat shock protein 70
  • PF11_0364 conserved Plasmodium protein, unknown function
  • PF13_0021 small heat shock protein, putative
  • PF13_0033 26S proteasome regulatory subunit, putative
  • PF13_0065 (Vacuolar ATP H+ Synthase subunit a) vacuolar ATP synthase subunit a
  • PF13_0082 cop-coated vesicle membrane protein p24 precursor, putative
  • PF13_0119 (Rab11A, Rab11) Rab GTPase 11a
  • PF13_0130 vacuolar ATP synthase subunit g, putative
  • PF13_0141 L-lactate dehydrogenase
  • PF13_0143 phosphoribosylpyrophosphate synthetase
  • PF13_0238 kelch protein, putative
  • PF13_0252 (NT1, ENT1) nucleoside transporter 1
  • PF13_0272 thioredoxin-related protein, putative
  • PF13_0346 60S ribosomal protein L40/UBI, putative
  • PF14_0075 (PM IV, PM4) plasmepsin IV
  • PF14_0076 (FAF-2, aspartic hemoglobinase I, PMI, PM1, Plasmepsin I, PM I) plasmepsin I
  • PF14_0077 (PM II, PM2, PMII, Plasmepsin II) plasmepsin II
  • PF14_0078 (HAP) plasmepsin III,histo-aspartic protease
  • PF14_0102 (RAP-1 p86, RAP-1 p82, RAP-1 p67, RAP1, RAP-1) rhoptry-associated protein 1
  • PF14_0105 conserved Plasmodium protein, unknown function
  • PF14_0124 actin II
  • PF14_0329 conserved protein, unknown function
  • PF14_0359 HSP40, subfamily A, putative
  • PF14_0361 secretory complex protein 62
  • PF14_0393 structure specific recognition protein, putative
  • PF14_0401 tRNA binding protein, putative
  • PF14_0425 (aldolase, pfAldolase) fructose-bisphosphate aldolase
  • PF14_0439 (LAP) M17 leucyl aminopeptidase
  • PF14_0517 (APP) aminopeptidase P
  • PF14_0541 (VP1, V-type H(+)-translocating pyrophosphatase) V-type H( )-translocating pyrophosphatase, putative
  • PF14_0543 (SPP) signal peptide peptidase
  • PF14_0598 (pfGAPDH) glyceraldehyde-3-phosphate dehydrogenase
  • PF14_0615 ATP synthase (C/AC39) subunit, putative
  • PF14_0655 helicase 45
  • PF14_0678 (EXP2, EXP-2) exported protein 2
  • PFA0110w (RESA) ring-infected erythrocyte surface antigen
  • PFB0065w stevor
  • PFB0210c (HT1) hexose transporter
  • PFB0395w conserved Plasmodium protein, unknown function
  • PFB0595w heat shock protein 40, putative
  • PFD0810w (Sar1p, Sar1) small GTP-binding protein sar1
  • PFE0075c rhoptry-associated protein 3
  • PFE0080c (RAP2) rhoptry-associated protein 2
  • PFE0625w (Rab1b) Rab GTPase 1b
  • PFE1050w adenosylhomocysteinase,S-adenosyl-L-homocysteine hydrolase
  • PFE1150w (Pdh1, mdr1) multidrug resistance protein
  • PFE1195w (Kb) karyopherin beta
  • PFE1250w acyl-CoA synthetase, PfACS10
  • PFE1590w early transcribed membrane protein 5
  • PFF0435w ornithine aminotransferase
  • PFF0870w conserved Plasmodium membrane protein, unknown function
  • PFF1335c 4-methyl-5(B-hydroxyethyl)-thiazol monophosphate biosynthesis enzyme
  • PFI0155c (Rab7) Rab GTPase 7
  • PFI0265c (RhopH3, Rhop-3) high molecular weight rhoptry protein 3
  • PFI0345w GTPase activator, putative
  • PFI0630w 26S proteasome regulatory subunit, putative
  • PFI0720w transporter, putative
  • PFI0875w (grp, Hsp70-2, BiP) heat shock protein 70
  • PFI0880c (GAP50, SAP) glideosome-associated protein 50,secreted acid phosphatase
  • PFI1020c inosine-5'-monophosphate dehydrogenase
  • PFI1090w S-adenosylmethionine synthetase
  • PFI1105w phosphoglycerate kinase
  • PFI1270w conserved Plasmodium protein, unknown function
  • PFI1445w (RhopH2) high molecular weight rhoptry protein 2
  • PFI1475w (MSP1, MSP-1, msp1 unprocessed, msp1, Msp-1 19, MSP1 19) merozoite surface protein 1
  • PFI1670c vacuolar ATP synthase subunit e, putative
  • PFI1730w (CLAG9) cytoadherence linked asexual protein 9
  • PFL0740c (Cpn10) 10 kd chaperonin
  • PFL1070c endoplasmin homolog precursor, putative
  • PFL1500w (Rab2) Rab GTPase 2
  • PFL1545c (Cpn60-2) chaperonin, cpn60
  • PFL1725w ATP synthase beta chain, mitochondrial precursor, putative
  • PFL1825w conserved Plasmodium membrane protein, unknown function
  • PFL2215w (actin I) actin I