ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

Francia, M. E., Wicher, S., Pace, D. A., Sullivan, J., Moreno, S. N., Arrizabalaga, G.
A Toxoplasma gondii protein with homology to intracellular type Na/H exchangers is important for osmoregulation and invasion. (2011 Jun 10, Exp Cell Res) PubMed / full text
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• "TgVP1 has been shown to partly co-localize with a cathepsin l protease (TgCPL) to the PLV/VAC [14] and [15]."
• Microscopy type: fixed light
• Microscopy method: antibody
• Strain: RH HPT-
• Gene model mapping comments: inferred from another publication
• Localisation record: PLV/VAC during intracellular tachyzoite and extracellular tachyzoite
• Other genes localised in this publication: sodium/hydrogen exchanger, putative, H+-translocating inorganic pyrophosphatase TVP, putative

Parussini, F., Coppens, I., Shah, P. P., Diamond, S. L., Carruthers, V. B.
Cathepsin L occupies a vacuolar compartment and is a protein maturase within the endo/exocytic system of Toxoplasma gondii. (2010 Jun, Mol Microbiol) PubMed / full text
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• "TgCPL localization in formaldehyde fixed RH parasites by immunofluorescence using MαTgCPL showing that TgCPL occupies a single apical localization in extracellular (i) and newly invaded parasites (ii). In contrast, TgCPL showed a punctate distribution in tachyzoites undergoing intracellular replication (iii)."
• Microscopy type: light, EM
• Microscopy method: monoclonal antibody against LARDEECRAQSCEKVV
• Strain: RH
• Gene model mapping comments: inferred from another publication
• Localisation record: apical during extracellular tachyzoite and newly invaded tachyzoite, vac during tachyzoites undergoing intracellular replication
• Comment: vac is an acronym for vacuolar compartment
• Other genes localised in this publication: Rhoptry kinase family protein ROP2A (incomplete catalytic triad), Rhoptry kinase family protein ROP4 / ROP7 (incomplete catalytic triad) ***WARNING: GENE MODEL INACCURATE ***, gorasp2-prov protein, dynamin-like protein, putative, apical membrane antigen 1, putative, Rab 5, H+-translocating inorganic pyrophosphatase TVP, putative, Ras family domain-containing protein, MIC2-associated protein M2AP, caltractin, putative

Larson, E. T., Parussini, F., Huynh, M. H., Giebel, J. D., Kelley, A. M., Zhang, L., Bogyo, M., Merritt, E. A., Carruthers, V. B.
Toxoplasma gondii cathepsin L is the primary target of the invasion-inhibitory compound morpholinurea-leucyl-homophenyl-vinyl sulfone phenyl. (2009 Sep 25, J Biol Chem) PubMed / full text
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• "As shown by fluorescently-labeled LHVS and TgCPL-specific antibodies, TgCPL is associated with a discrete vesicular structure in the apical region of extracellular parasites, but is found in multiple puncta throughout the cytoplasm of intracellular replicating parasites."
• Microscopy type: Light
• Microscopy method: polyclonal antibody directly to protein
• Strain: RH
• Gene model mapping comments: Blast from DQ407191, annotation matches
• Localisation record: rhoptry and posterior to nucleus during intracellular tachyzoite and extracellular tachyzoite
• Other genes localised in this publication: -

Huang, R., Que, X., Hirata, K., Brinen, L. S., Lee, J. H., Hansell, E., Engel, J., Sajid, M., Reed, S.
The cathepsin L of Toxoplasma gondii (TgCPL) and its endogenous macromolecular inhibitor, toxostatin. (2009 Mar, Mol Biochem Parasitol) PubMed / full text
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• "TgCPL was found primarily in the apical end of the tachyzoite (Fig. 6A), but did not co-localize with any apical organelles, including rhoptries, dense granules, or micronemes (data not shown)."
• Microscopy type: Light, EM
• Microscopy method: polyclonal antibody directly to protein
• Strain: RH
• Gene model mapping comments: Blast from AF184984
• Localisation record: apical during tachyzoite
• Other genes localised in this publication: -