ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

No assigned gene identifier (PRX, PRX1, 49.m03355, OPN, Der1-2ER, Der1Ap, RNG1)

A gene from Toxoplasma gondii

Compiled localisation
cytoplasm during extracellular tachyzoite and intracellular tachyzoite
apicoplast periphery and conoid and not apicoplast lumen and ring structure at apical inner membrane complex
apical and parasitophorous vacuole membrane during tachyzoite
apicoplast and endoplasmic reticulum during 24 hours after tachyzoite infection

Who localised this protein by microscopy?

Tran, J. Q., de Leon, J. C., Li, C., Huynh, M. H., Beatty, W., Morrissette, N. S.
RNG1 is a late marker of the apical polar ring in Toxoplasma gondii. (2010 Sep, Cytoskeleton (Hoboken)) PubMed / full text
   more detail about this publication

ring structure at apical inner membrane complex
  • "The Toxoplasma 49.m03355 gene encodes a small, low complexity protein that localizes to an apical ring."
  • Microscopy type: light, EM
  • Microscopy method: YFP tag, mCherry tag
  • Strain: RH
  • Gene model mapping comments: Taken directly from publication
  • Localisation record: apical ring
  • Other genes localised in this publication: hypothetical protein

Huynh, M. H., Carruthers, V. B.
Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80. (2009 Apr, Eukaryot Cell) PubMed / full text
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conoid

Agrawal, S., van Dooren, G. G., Beatty, W. L., Striepen, B.
Genetic evidence that an endosymbiont-derived endoplasmic reticulum-associated protein degradation (ERAD) system functions in import of apicoplast proteins. (2009 Nov 27, J Biol Chem) PubMed / full text
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apicoplast and endoplasmic reticulum during 24 hours after tachyzoite infection, apicoplast periphery and not apicoplast lumen

Cortez, E., Stumbo, A. C., Saldanha-Gama, R., Villela, C. G., Barja-Fidalgo, C., Rodrigues, C. A., das Gracas Henriques, M., Benchimol, M., Barbosa, H. S., Porto, L. C., Carvalho, L.
Immunolocalization of an osteopontin-like protein in dense granules of Toxoplasma gondii tachyzoites and its association with the parasitophorous vacuole. (2008, Micron) PubMed / full text
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apical and parasitophorous vacuole membrane during tachyzoite
  • "Our results also showed, after macrophage invasion, an intense positive labeling for OPN-like protein at the sub-apical portion of tachyzoites, the site of dense granules secretion, and the localization of this protein at the parasitophorous vacuole membrane."
  • Microscopy type: Light, EM
  • Microscopy method: monoclonal antibody to homologue
  • Strain: RH
  • Gene model mapping comments: inferred from another publication
  • Localisation record: apical and PVM during tachyzoite
  • Comment: Blast from mouse genbank AF515708 shows no hit in the genome, but has much more significant hit in the assembly. No gene model or sequence mentioned in the paper
  • Other genes localised in this publication: -

Son, E. S., Song, K. J., Shin, J. C., Nam, H. W.
Molecular cloning and characterization of peroxiredoxin from Toxoplasma gondii. (2001 Jun, Korean J Parasitol) PubMed / full text
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cytoplasm during extracellular tachyzoite and intracellular tachyzoite
  • "TgPrx was located in the cytoplasm of T. gondii extracellularly or intracellularly by immunofluorescence assay."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: RH
  • Gene model mapping comments: blast from AY043228, no correct gene model – see AY633703
  • Localisation record: cytoplasm during intracellular tachyzoite and extracellular tachyzoite
  • Comment: gene model TGME49_066120 should be 3 different gene models, so not recorded
  • Other genes localised in this publication: -

Orthology

OrthoMCL

No entry found in OrthoMCL.

Amino Acid Sequence

amino acid sequence not recorded

Gene-Specific Links for A common gene for all genes not assigned to a gene model

General Sub-Cellular Localisation Links

  • TargetP, prediction of signal peptides, as well as chloroplast and mitochondrial transit peptides
  • OrthoMCL, automatic clustering of orthologous groups of proteins