ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

PFC0105w (SRPK1, CLK-4)

serine/threonine protein kinase, putative, a gene from Plasmodium falciparum

Compiled localisation
nucleus during ring and trophozoite
cytoplasm during gametocyte and schizont and trophozoite
not nucleus during gametocyte and schizont

Who localised this protein by microscopy?

Dixit, A., Singh, P. K., Sharma, G. P., Malhotra, P., Sharma, P.
PfSRPK1, a novel splicing-related kinase from Plasmodium falciparum. (2010 Dec 3, J Biol Chem) PubMed / full text
   more detail about this publication

nucleus during ring and trophozoite, cytoplasm during gametocyte and schizont and trophozoite, not nucleus during gametocyte and schizont
  • "These proteins were present predominantly inside the nucleus in the ring stages as indicated by co- localization with nuclear dye DAPI (Fig. 6B). PfSRPK1 and PfSR1 were present in the nucleus of ~75% of rings and young trophozoites that were analyzed. As the parasites matured, PfSRPK1 and PfSR1 seemed to be exported to nuclear periphery and/or cytoplasm in mature trophozoites. Both these proteins were predominantly in the cytoplasm of most (>80%) schizonts. Interestingly, both PfSRPK1 and PfSR1 are present in the cytoplasm of gametocytes with possibly only a small fraction in the nucleus (Fig. 6B). "
  • Microscopy type: Fixed Light
  • Microscopy method: antibody to NVNKINCKVINKKNS
  • Strain: 3D7
  • Gene model mapping comments: inferred from another publication
  • Localisation record: Predominantly nucleus during ring, nuclear periphery and cytoplasm during trophozoite, cytoplasm and not nucleus during schizont, cytoplasm and not nucleus during gametocyte
  • Other genes localised in this publication: Ser/Arg-rich splicing factor, putative, survival of motor neuron (SMN)-like protein

Orthology

OrthoMCL

Part of OrthoMCL group OG4_10603

Localised Genes in this Group
Other Apicomplexan Genes in this Group
Non-Apicomplexan Genes in this OrthoMCL Group with Gene Ontology 'Inferred from Direct Assay (IDA)' Cellular Component Terms

Amino Acid Sequence

>PFC0105w serine/threonine protein kinase, putative
MSFSNTCSLSNNSNSSSSSEDATSGKLQYTESDDEGSDEYCEGGYHPVKINEIYNDRYRIEGKLGWGHFSTVWVATDLKS
KPLKFVAIKIQKGSETYTESAKCEINYLNTVKVNSFDSSWVELKEQQRERLFHYNMTKGVVSFIDSFEHKGPNGTHICMV
FEFMGPNLLSLIKHYDYKGIPLNLVRKIATHVLIGMQYLHDVCKIIHSDIKPENVLVSPLTTIPKPKDYTKDKLESNKSN
QVEKKENDQNVDKKLITTMNNNINTNLSEKKKVINDTQKNDKNIEYDQKCTSSKENIEDNVSFVNDPSDPNQKNNLNNNI
TDNNIIPSNVQIEKQSTLSKNKKNEKDSYININNSLTNDDQNLKREDIKFNDKAEGITKYDMLNIKNNISIKEKINDCHS
PNENKNKDNHNQCEDNSINICNNKNNNIQTNNINDNTVNEKINNTSKKDMLNNTQNNNDSEKNDVVIEQQLVNEDILKKK
NKQTKKKKNINEPPYVKHKLRPSNSDPSLLTSYSNIHALQETLTRKPYHYNTYFLNNPEKYRDNKMNPYLHRLPNDCLKK
IDQDDSDETEEEDDLSDVDQNKEQNKNQLEVNLPNNKYPNSNDVYKFFEKDINKFPIYCDMFNHLIHPEALRLHELYMKN
KKNIDSNNTMNDLGNNQNSHKVVYINTEDGEYCIRPYDPSVYYHEKSCYKICDLGNSLWIDESRYAEIQTRQYRAPEVIL
KSGFNETADIWSFACMVFELVTGDFLFNPQKGDRYDKNEEHLSFIIEVLGNIPKHMIDAGYNSHKYFNKNNYRLKNIRNI
KKYGLYKILKYKYNLPEKEISPLCSFLLPMLSVDPQTRPSAYTMLQHPWLNMVSLEEGDDMYINDESYSINNDRNMKNNS
NSNNFIYDGHNSSKNKNSSNKKKIDVNYKIGNNGNNAYNDNYYNKNYKNNKNNKNFNDDVVEPSPDQYMHANYNNDIVHA
VLYEKPYNSNNVISYTNNKGHKNNFDINYLQHRNDNNSNKQNISLTTNDYTFNSDYIANMMDHDTYRKQIIKNIPAHQIS
KLKDGKNFKAYNESIQYEMHDFQQYNEHDFEYKFNKRFEHAHHIKEMKHNDDDYEEEDEDEDDDDEDYESDVDYDDDDEY
DEGQEHDADQDEKNNDNEKQQEQQNYGEKYNYEHYENNMGYNKNIQQLSYTNNNDDENNFCETQNIYILQNKRDINFKEC
TPRNNINKEIKSDKYQSSKVINQKDNYWNYKIKENTKLREHAKKQHYSNNNNINKNDNTNIMNQIDTKDQISKNLHDLST
NNNMDQKHGALQKMHMNEKTNQDKPLNDEEILIENRDDQNVNKINCKVINKKNSCAYT

Gene-Specific Links for PFC0105w

General Sub-Cellular Localisation Links