ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

PF14_0723 (CCp1)

LCCL domain-containing protein, a gene from Plasmodium falciparum

Compiled localisation
beyond erythrocyte membrane and cytoplasm and parasitophorous vacuole and proximal to plasma membrane during mature gametocyte
during gametocyte stage ii and gametocyte stage iii and gametocyte stage iv and gametocyte stage v and not gametocyte stage i and not intraerythrocytic and not male gametocyte
parasite plasma membrane during emerging male gametocyte and female gametocyte and gametocyte stage v and mature gametocyte
erythrocyte during gametocyte stage i and gametocyte stage ii
not parasite plasma membrane during male gametocyte

Who localised this protein by microscopy?

Scholz, S. M., Simon, N., Lavazec, C., Dude, M. A., Templeton, T. J., Pradel, G.
PfCCp proteins of Plasmodium falciparum: gametocyte-specific expression and role in complement-mediated inhibition of exflagellation. (2008 Mar, Int J Parasitol) PubMed / full text
   more detail about this publication

during gametocyte stage ii and gametocyte stage iii and gametocyte stage iv and gametocyte stage v and not gametocyte stage i, erythrocyte during gametocyte stage i and gametocyte stage ii, parasite plasma membrane during emerging male gametocyte and female gametocyte, not parasite plasma membrane during male gametocyte
  • "PfCCp1, PfCCp2 and PfCCp3 are expressed in a punctate pattern, as has been reported ([Delrieu et al., 2001, [Pradel et al., 2004 and [Pradel et al., 2006). No protein expression was detectable in asexual parasites for PfCCp1 through PfCCp4 and PfFNPA (Fig. 1b and Supplementary Fig. S1). With the exception of PfCCp4, which can be detected as early as stage I gametocytes (Fig. 2b), all proteins are initially expressed in stage II gametocytes. At this stage, PfCCp labelling was also found highlighting infected host erythrocytes, although erythrocyte labelling is no longer observed by gametocyte stage III (Fig. 2a). Expression for all six proteins persists during gametocyte maturation. After gametocyte emergence PfCCp1 through PfCCp3, PfCCp5 and PfFNPA revealed a patchy expression pattern on the plasma membrane of macrogametes, while PfCCp4 is still associated homogenously with this membrane (Fig. 2a). PfCCp protein expression decreased in Pfs25-positive zygotes or unfertilized macrogametes by 24 h after emergence (data not shown). While PfCCp labelling can sometimes be observed on the surface of emerging microgametocytes, PfCCp protein is not present on the surface of exflagellating microgametes."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: NF54
  • Gene model mapping comments: Blast from NP_702612, annotation matches, taken directly from publication
  • Localisation record: Punctate during stage II gametocyte and stage III gametocyte and stage IV gametocyte and stage V gametocyte, sometimes erythrocyte during stage I gametocyte and stage II gametocyte, not during stage I gametocyte, patchy plasma membrane during macrogamete, sometimes surface during emerging microgametocyte, not surface during microgamete
  • Comment: surface during male gametocyte and macrogamete, not during microgamete, not during stage I gametocyte
  • Other genes localised in this publication: LCCL domain-containing protein, LCCL domain-containing protein, LCCL domain-containing protein, LCCL domain-containing protein, LCCL-like protein, 25 kDa ookinete surface antigen precursor

Pradel, G.
Proteins of the malaria parasite sexual stages: expression, function and potential for transmission blocking strategies. (2007 Dec, Parasitology) PubMed / full text
   more detail about this publication

parasite plasma membrane during gametocyte stage v
  • "(B) Punctuate expression of PfCCp1 associated with the gametocyte stage V surface (PfCCp1 labelling shown in green, erythrocyte shown in red)."
  • Microscopy type: Light
  • Microscopy method: antibody
  • Strain:
  • Gene model mapping comments: inferred from another publication
  • Localisation record: punctate surface during stage V gametocyte
  • Comment: strain information not found
  • Other genes localised in this publication: 6-cysteine protein, alpha tubulin 2, LCCL domain-containing protein, 25 kDa ookinete surface antigen precursor

Pradel, G., Hayton, K., Aravind, L., Iyer, L. M., Abrahamsen, M. S., Bonawitz, A., Mejia, C., Templeton, T. J.
A multidomain adhesion protein family expressed in Plasmodium falciparum is essential for transmission to the mosquito. (2004 Jun 7, J Exp Med) PubMed / full text
   more detail about this publication

beyond erythrocyte membrane and cytoplasm and parasite plasma membrane and parasitophorous vacuole and proximal to plasma membrane during mature gametocyte, during not intraerythrocytic and not male gametocyte
  • "For PfCCp1, PfCCp2, and PfCCp3, a punctate rim fluorescence pattern was observed in P. falciparum gametocytes, whereas asexual stage parasites did not show any labeling (Fig. 4 B). Immunogold labeling revealed localization of PfCCp1 associated with the parasite surface membrane in the parasitophorous vacuole of mature gametocytes (Fig. 5, A and C). Figure 5. Ultrastructural PfCCp1 localization associates with the parasite membrane in the parasitophorous vacuole. Immunoelectron microscopic examination using anti-PfCCp1 primary antibody and either immunogold (A, enlarged in C) or alkaline phosphatase (B, enlarged in D) secondary labeling localize expression to the parasitophorous vacuole of mature gametocytes as well as in the gametocyte cytoplasm and attached to the outside of erythrocyte membranes."
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: NF54
  • Gene model mapping comments: inferred from another publication
  • Localisation record: punctate rim during mature gametocyte, not during asexual, parasite surface during mature gametocyte, PV and cytoplasm and outside of erythrocyte membranes during mature gametocyte, not during microgamete
  • Other genes localised in this publication: LCCL domain-containing protein, LCCL domain-containing protein

Orthology

OrthoMCL

Part of OrthoMCL group OG4_21070

Localised Genes in this Group
Other Apicomplexan Genes in this Group
Non-Apicomplexan Genes in this OrthoMCL Group with Gene Ontology 'Inferred from Direct Assay (IDA)' Cellular Component Terms
    There are no non-apicomplexan genes that have manually annotated evidence codes.

Amino Acid Sequence

>PF14_0723 LCCL domain-containing protein
MHHLLFIIWYIILNYYVSGQESATNFYKFIDSFASSTYMSEESGSSAYDAKRAIQNNPNYWCSSGNHSNDEEITWTGYLN
TKGFIKGVKVSWAYSPEFVKISVSSDGEKYRTIIPYKKISSNEASFDEIYFFKRLEEAMSIKIGLKNARHKYFGIREVKL
IGGGNPYFLLLSGISSEEEMCLQVEEGLINNDNTSIILDSCTNALASGDGRELWKTNSNNQVISAFSDPPKCLSVVNLDD
LENNKIVLYDCLRALEDGDGKSNWIFESNSQIRLQKSGDAFCISQKNIYGNIPGIHDILLNLDVSIYSNSTLDDDHNPDN
TIDGNLNSYWASATFTDNYDHLVYLVLDLNKITDLSRIKIYWEYPPLHYNISVSTDNQNFTVVSENLANPSYITVDSLKN
METRYIKISMIKTHPKHGELGDNFLYGIRSIEVQANNLETVINHCRDAANSDDARDKYFVEYITEFDKDLTNKLINLEDD
VTKNVSSISDNLSKLEELLPNIETCLEEKKTYDEELKESKEKANDLNNKLSLLTSVNVNTLDSDILKLGILPGDSYNFPA
NDCAVIKNVQENPLSGFYWIKPKCSPEPLRVYCDMDSSTSIYIWNGNPPKSPDHLITNMINSVNDIRQHCAEVGLQPLIL
RSKNQLNSLIISLKKIGYSLNGKVNIPLAYDYSCDHGSCSGRFHDLLNGNIDISTLIYLKASESPDSTKVRQTAGISYDD
GSFKFFNLETSDISAIVCSTNSTENDSALQYLSINCETTGMEDSFHSIVNTNIVVLCPLGCDDEKYHDASIYGSRGTYSD
NSSICRAAIHSDIIDNKGGLVNVTIESGMDHYVGSINNNIESISLNKNEKGLLDIIPEEKEGTNNIREESSIFHHKTIRV
SSLIEDCPLDLFLFNQTSFLEKGNNIRNNKGTELKYNDDENMTVKNFHELISNLMENIDAIHGVDSSVISIVQEETIRII
EKTKKELKPADMLSKKQIEDAMNLYNLTENLAIYLYDLSSKYIQDLEKLKNTLEELKGAQKVAHNFGTFKLNYETMNFST
HFSLFDSNLIKNKESVWGYSDTNILGHENSIGQMNSVSSQEIGEGYYAKLKGLNFYDFDFNISVLSRGTGCLGVVFRAKD
DFNFYLFDICDKDGTKRLSKVENGQVHILKKVVNSDVTLNNQWNKYKIITKHANIDIYEVDKDNNMIKILSSLDERFLSG
TVGLYSQIYGLGTFFDDLEVIALPCTQLSELNTLNKNVKSNCPYYKENYLNNLMSYDIIYNPNNYFNWNVEKENEQNYLL
CSKNEEEVKNAKDEKDIYTIVLLKLRECTDGTFNFDIQVSDDETGNISKKLSYIYILFHYKDENNFNALEMKDGKLAFLT
NKNGKSFILSERNEEENDNNKNIEKRFTFVQNEWIHVNLHFDKSTFKVIIITNNNEDKFVLSAKSRNDVPLGKVGFLVHN
FDEVKFDSILLNSPTITKVDENFLQVKSKTWANCEDSVHVLHRRFSCETDIYPNETKEKHIKCIKNFCKECCLYHTQLLD
SNEKNECEKHCKQNDNLAAKMQTLFEKFINRCVSLNENEDYETCDKNDKKCKNKVCVLCCKKHDPTTSKELKVLPMNQFK
KIQENEIIECQLQCNMIHSI

Gene-Specific Links for PF14_0723

General Sub-Cellular Localisation Links