ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

PF14_0532 (CCp2)

LCCL domain-containing protein, a gene from Plasmodium falciparum

Compiled localisation
proximal to plasma membrane during mature gametocyte
during gametocyte stage ii and gametocyte stage iii and gametocyte stage iv and gametocyte stage v and not gametocyte stage i and not intraerythrocytic
erythrocyte during gametocyte stage i and gametocyte stage ii
parasite plasma membrane during emerging male gametocyte and female gametocyte
not parasite plasma membrane during male gametocyte

Who localised this protein by microscopy?

Scholz, S. M., Simon, N., Lavazec, C., Dude, M. A., Templeton, T. J., Pradel, G.
PfCCp proteins of Plasmodium falciparum: gametocyte-specific expression and role in complement-mediated inhibition of exflagellation. (2008 Mar, Int J Parasitol) PubMed / full text
   more detail about this publication

during gametocyte stage ii and gametocyte stage iii and gametocyte stage iv and gametocyte stage v and not gametocyte stage i, erythrocyte during gametocyte stage i and gametocyte stage ii, parasite plasma membrane during emerging male gametocyte and female gametocyte, not parasite plasma membrane during male gametocyte
  • "PfCCp1, PfCCp2 and PfCCp3 are expressed in a punctate pattern, as has been reported ([Delrieu et al., 2001, [Pradel et al., 2004 and [Pradel et al., 2006). No protein expression was detectable in asexual parasites for PfCCp1 through PfCCp4 and PfFNPA (Fig. 1b and Supplementary Fig. S1). With the exception of PfCCp4, which can be detected as early as stage I gametocytes (Fig. 2b), all proteins are initially expressed in stage II gametocytes. At this stage, PfCCp labelling was also found highlighting infected host erythrocytes, although erythrocyte labelling is no longer observed by gametocyte stage III (Fig. 2a). Expression for all six proteins persists during gametocyte maturation. After gametocyte emergence PfCCp1 through PfCCp3, PfCCp5 and PfFNPA revealed a patchy expression pattern on the plasma membrane of macrogametes, while PfCCp4 is still associated homogenously with this membrane (Fig. 2a). PfCCp protein expression decreased in Pfs25-positive zygotes or unfertilized macrogametes by 24 h after emergence (data not shown). While PfCCp labelling can sometimes be observed on the surface of emerging microgametocytes, PfCCp protein is not present on the surface of exflagellating microgametes."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: NF54
  • Gene model mapping comments: Blast from NP_702421, annotation matches, taken directly from publication
  • Localisation record: Punctate during stage II gametocyte and stage III gametocyte and stage IV gametocyte and stage V gametocyte, sometimes erythrocyte during stage I gametocyte and stage II gametocyte, not during stage I gametocyte, patchy plasma membrane during macrogamete, sometimes surface during emerging microgametocyte, not surface during microgamete
  • Other genes localised in this publication: LCCL domain-containing protein, LCCL domain-containing protein, LCCL domain-containing protein, LCCL domain-containing protein, LCCL-like protein, 25 kDa ookinete surface antigen precursor

Pradel, G., Hayton, K., Aravind, L., Iyer, L. M., Abrahamsen, M. S., Bonawitz, A., Mejia, C., Templeton, T. J.
A multidomain adhesion protein family expressed in Plasmodium falciparum is essential for transmission to the mosquito. (2004 Jun 7, J Exp Med) PubMed / full text
   more detail about this publication

proximal to plasma membrane during mature gametocyte, during not intraerythrocytic
  • "For PfCCp1, PfCCp2, and PfCCp3, a punctate rim fluorescence pattern was observed in P. falciparum gametocytes, whereas asexual stage parasites did not show any labeling (Fig. 4 B)."
  • Microscopy type: Light
  • Microscopy method: polyclonal antibody directly to protein
  • Strain: NF54
  • Gene model mapping comments: inferred from another publication
  • Localisation record: punctate rim during mature gametocyte, not during asexual
  • Other genes localised in this publication: LCCL domain-containing protein, LCCL domain-containing protein

Orthology

OrthoMCL

Part of OrthoMCL group OG4_22401

Localised Genes in this Group
Other Apicomplexan Genes in this Group
  • PBANKA_131950 LCCL domain-containing protein CCP2
  • PY01580 LCCL domain-containing protein CCP2
  • PY05090 LCCL domain-containing protein CCP2-related
  • PVX_117675 LCCL domain-containing protein CCP2, putative
  • PCHAS_132280 LCCL domain-containing protein CCP2, putative
  • PKH_125030 hypothetical protein, conserved in Apicomplexan species
  • TGME49_005660 F5/8 type C domain-containing protein
  • NCLIV_020010 F5/8 type C domain-containing protein, putative
  • cgd7_300 CpCCP2; extracellular protein with a signal peptide, ricin, discoidin, NEC, LCCL, 2 levanase and an apicomplexan-specific cysteine-rich repeat (apicA repeat)
  • Chro.70043 hypothetical protein
  • CMU_006600 LCCL domain-containing protein
  • TA07240 hypothetical protein, conserved
  • BBOV_II003700 LCCL domain-containing protein CCP2, putative
Non-Apicomplexan Genes in this OrthoMCL Group with Gene Ontology 'Inferred from Direct Assay (IDA)' Cellular Component Terms
    There are no non-apicomplexan genes that have manually annotated evidence codes.

Amino Acid Sequence

>PF14_0532 LCCL domain-containing protein
MTKLFFINYAFVIIFSFNLFVKCGDDISNTFFKFEFCEATSTFSSIGENGLPQYAAENALTRGSGYWCSEGKHNVNDVVS
WIGHLKNVRSLNGIIIHWAYTPGEVSILASYDGNEPYEEVVPYQLLESRVGNVVQNIIFNHVIRAKSIKVNMRHAIHDYF
GINFTNVLGSRDPTLRIQSGMSSLTQDLCLQIDEKNEVVLDGCITAISYLDGRDLWKLNSKNQIYNPINNLCITLKDNLI
ANGGRLILEDCNASLEHNDGRSSWQLLPNNQLKILRNGNFCLSQDGHKSGSIDVAFHKECTSTLSSDNKNHSPDKVVDGL
LDTFWVSQEFNLDTAPDSVHFDVNLGSIYKLQKAIIDWKYPATKYSISLSNDGENYKEVSSNLANFLRSTINNLHNTEAQ
YIRLTLMAPNPEFSEENKLFYGIKKFSVYSNRIKSIVDDCDKIKDTDDARDKYFFEFVSEVNLQEGKELKRLDNELQLYA
EKIQNEALKIQSLNPKLKKCKLEKEKRHKDISNIKNVILKNIYEVIKQTENIIKMNPLSSYYSTSTKELGQTSDNPADNC
FHLKNALPSSPSGFYYVLTTCSQNVLRVFCDMKMGATYYIPSVDNKIINKLKDVENVCATYGLNPIHLYHESQIYTLRKV
FDTMDINITNPVPLAIRKEDSEFYYSLDFQTNVHDIIAKFGTPVGNTFGINNIGITFFDSSSSEMSAFVCSDNINSINLP
EPFVNLDCQSSLKETNEIEKMIGNEYLIKCPHDCLERDIEESVIGGEGNIYSEDSSICLSAIHAGIYDKHYLIHLRVINA
LNEYGGFFQNGIISESFFNNTQEVGFKLFHVPPKCPKDDITSNINNNNNYYYYDNNNSNAMFSFLELDNKMNNVNDKFDN
NDYTYVDSSTADAINDLITIVNKQVGSTDTTFLALINKQSIKIISNARRYLKPTEIFEKNIELLSNETLKDVEKVFNLIK
VLSSKINSELEKKKYKLEILVDERLRQKEFESWKLDNIDNIYDTFEIINSVQLQQIGKWNILDNPLYEGINGITLIQNVR
VYNSPENSVINSFNGSYAFLRYKSFYDFVFSTYVNIKGVGSVGLIFRSYDKYNFYMLELNNDRQKNEFNKRLLKFENNIV
TELAIVNGNDLQEGDWFVVRIECIGSKIIITVLKTNKPIYELPKPDIIINDDFTSSGTIGFYTYGIDNVQFTNITVESVE
CSTKEILSYNISPISCNIYEEYYVGKFNKSYIPFDSENSNSGSSNWKFAKNIGNEKHVILQNSNMKQIENEEQIPSFIIL
QNKSCQTGVLNFSVYPECSNGIVGTMFKFLDSKNYTILEIGSGFTRLRQNVNGKFQLLSKSIISGYKEHIWNRVTVSFSS
NNINVNLGTGFMTYPIFSLIGLHLSDGESVGFTSYNCSNVSFSNIYMHPFDFKPYTPTPTLDTESFLPPIFSKFDQATIK
EEDQSQDMGYKQIGDNKNSDISKDSPIDKHSFEDSTRQMKKDAYYCATHKNIVDIINYCNQYDKENDNCTNEFCTICCNN
IDTKEEEDIRTCEILCQKLDDKILQTSEVLNYLKKSCIESPNEELKKSCEDDNDKEECLIEMCEMCCQSVTIPDDLLTSH
MDIDSLTNHCISLCDKP

Gene-Specific Links for PF14_0532

General Sub-Cellular Localisation Links