liver stage antigen 1, a
gene from Plasmodium falciparum
parasitophorous vacuole during hepatocyte stage day 3 and hepatocyte stage day 5
not cytoplasm and not parasite plasma membrane during hepatocyte stage day 3 and hepatocyte stage day 5 and hepatocyte stage day 7
around cytomeres during hepatocyte stage day 7
during not intraerythrocytic and not sporozoite
Who localised this protein by microscopy?
Fidock, D. A., Gras-Masse, H., Lepers, J. P., Brahimi, K., Benmohamed, L., Mellouk, S., Guerin-Marchand, C., Londono, A., Raharimalala, L., Meis, J. F., et, a. l. ..
parasitophorous vacuole during hepatocyte stage day 3 and hepatocyte stage day 5, not cytoplasm and not parasite plasma membrane during hepatocyte stage day 3 and hepatocyte stage day 5 and hepatocyte stage day 7, around cytomeres during hepatocyte stage day 7, during not intraerythrocytic and not sporozoite
Plasmodium falciparum liver stage antigen-1 is well conserved and contains potent B and T cell determinants. (1994 Jul 1, J Immunol)
more detail about this publication
"Initially, in day 3 (i.e., four- to eight-nuclei stage) and small day 5 forms (Fig. 3, a and b, respectively), the LSA-1 protein is located in the parasitophorous vacuole (PV) space, which is delineated by the inner plasmalemma and the outer PV membrane (PVM) and which forms a distinct ring separating the parasite cytoplasm from the host hepatocyte. At a later stage, the Ag appears also to infiltrate the spaces between the pseudocytomeres of the developing schizonts, as the plasmalemma forms deep invaginations into the parasite cytoplasm (Fig. 3c), and is subsequently seen localized around the cytomeres, just before the individualization of merozoites (Fig. 34. The ultrastructural localization of LSA-1 was revealed by immunogold labeling of fully mature forms, which showed this protein to be associated with the fluffy or flocculent material present in the PV that surrounds the emerging exo-erythrocytic merozoites (Fig. 3e). These Abs were repeatedly negative, by IFAT and Western analysis, on both blood stage and sporozoites."
Microscopy type: Light, EM
Microscopy method: polyclonal antibody directly to protein
Gene model mapping comments: Blast from Z30319, annotation matches, blast from Z30320
Localisation record: PV and not parasite cytoplasm and not surface during hepatocyte stage day 3 and hepatocyte stage day 5, around cytomeres and not cytoplasm and not surface during hepatocyte stage day 7, not during asexual and sporozoite
- Other genes localised in this publication:
Part of OrthoMCL group OG4_33463
Localised Genes in this Group
Other Apicomplexan Genes in this Group
There are no other apicomplexan genes.
Non-Apicomplexan Genes in this OrthoMCL Group with Gene Ontology 'Inferred from Direct Assay (IDA)' Cellular Component Terms
There are no non-apicomplexan genes that have manually annotated evidence codes.
Amino Acid Sequence
>PF10_0356 liver stage antigen 1
Not found in:
Gene-Specific Links for PF10_0356
General Sub-Cellular Localisation Links
Malaria Parasite Metabolic Pathways
MalSig, prediction of P. falciparum
PSEApred, prediction of P. falciparum
proteins secreted into the infected erythrocyte
PlasMit, prediction of P. falciparum
mitochondrial transit peptides
apicoplast leader predictors:
PlasmoDraft, predictions of P. falciparum gene function based on postgenomic data
TargetP, prediction of signal peptides, as well as chloroplast and mitochondrial transit peptides
OrthoMCL, automatic clustering of orthologous groups of proteins