PF16 protein, putative, a
gene from Plasmodium berghei
cytoplasm during before gametogenesis
cytoplasmic axonemes during 10 minutes after gamete activation and 5 minutes after gamete activation and after gamete emergence
during not macrogamete and not ookinete 24 hours after activation and not ookinete 4 hours after activation
Who localised this protein by microscopy?
Straschil, U., Talman, A. M., Ferguson, D. J., Bunting, K. A., Xu, Z., Bailes, E., Sinden, R. E., Holder, A. A., Smith, E. F., Coates, J. C., Rita, T. e. w. a. r. i.
cytoplasm during before gametogenesis, cytoplasmic axonemes during 10 minutes after gamete activation and 5 minutes after gamete activation and after gamete emergence, during not macrogamete and not ookinete 24 hours after activation and not ookinete 4 hours after activation
The Armadillo repeat protein PF16 is essential for flagellar structure and function in Plasmodium male gametes. (2010, PLoS One)
more detail about this publication
"Before the onset of gametogenesis, PbPF16-GFP appeared as diffuse cytoplasmic fluorescence (Figure 2). At 5 and 10 minutes after activation, more intense PbPF16 was seen associated with growing cytoplasmic axonemes (detected by ?-tubulin immunostaining; (Figure 2). Later, when male gametes were emerging from the residual body of the male gametocyte, PbPF16-GFP was clearly localised to the emerging male gamete and had patchy distribution along the length of the gamete; moreover it appeared that PbPF16-GFP was always encompassed within the axonemal fluorescence (Figure 2). The protein was not detected in activated female gametes or in the ookinete at either 4 hours or 24 hours after activation."
Microscopy type: Live Light
Microscopy method: GFP tag
Gene model mapping comments: taken directly from publication
Localisation record: diffuse cytoplasmic during before gametogenesis, cytoplasmic axonemes during 5 minutes after gamete activation, cytoplasmic axonemes during 10 minutes after gamete activation, axonemal during after gamete emergence, not during female gametes, not during ookinete 4 hours after activation, not during ookinete 24 hours after activation
- Other genes localised in this publication:
This gene does not have an OrthoMCL group.
Amino Acid Sequence
>PBANKA_091740 PF16 protein, putative
Not found in:
Gene-Specific Links for PBANKA_091740
General Sub-Cellular Localisation Links
TargetP, prediction of signal peptides, as well as chloroplast and mitochondrial transit peptides
OrthoMCL, automatic clustering of orthologous groups of proteins