inner membrane complex protein 1b, a
gene from Plasmodium berghei
pellicle during ookinete
not parasite plasma membrane and ookinete protrusion during retort
not peripheral of oocyst protrusion during oocyst protrusion
during early oocyst
Who localised this protein by microscopy?
Tremp, A. Z., Khater, E. I., Dessens, J. T.
pellicle during ookinete, ookinete protrusion and not parasite plasma membrane during retort, not peripheral of oocyst protrusion during oocyst protrusion, during early oocyst
IMC1b is a putative membrane skeleton protein involved in cell shape, mechanical strength, motility, and infectivity of malaria ookinetes. (2008 Oct 10, J Biol Chem)
more detail about this publication
"Here we have generated genetically modified parasites that express IMC1b tagged with green fluorescent protein, and we show that it is targeted exclusively to the pellicle of ookinetes. Very weak cytoplasmic fluorescence was observed in macrogametes and zygotes (data not shown). As the ookinete develops by forming a protrusion of the spherical zygote, a developmental stage called retort, strong fluorescence became localized to the periphery of the protrusion corresponding to the newly forming ookinete but not to the zygote part of the retort (Fig. 3). These observations argue against the targeting of IMC1b to the plasma membrane; in this scenario the protein would be localized to the periphery of entire retort, which is clearly not the case. We also studied the process of ookinete-to-oocyst transformation. This starts with the development of a round protrusion midway along the ookinete, a developmental stage called took. These protrusions were devoid of peripheral fluorescence (Fig. 3), indicating that IMC/SPN are absent from this part of the cell. As the rounding-up process of the ookinete completes, young spherical oocysts form; these displayed peripheral fluorescence only in part of the cell (Fig. 3). However, older multinucleate oocysts no longer displayed any fluorescence (data not shown) indicating loss of the entire IMC/SPN structures, consistent with documented oocyst architecture."
Microscopy type: Light
Microscopy method: GFP tag
Strain: ANKA clone 234
Gene model mapping comments: Blast from EAA15257, annotation matches, agrees with 'single exon, 535 residues long' as suggested in the publication
Localisation record: pellicle during ookinete, ookinete protrusion and not PM during retort, not peripheral of oocyst protrusion during oocyst protrusion, during young oocyst
- Other genes localised in this publication:
This gene does not have an OrthoMCL group.
Amino Acid Sequence
>PBANKA_090710 inner membrane complex protein 1b
Not found in:
Gene-Specific Links for PBANKA_090710
General Sub-Cellular Localisation Links
TargetP, prediction of signal peptides, as well as chloroplast and mitochondrial transit peptides
OrthoMCL, automatic clustering of orthologous groups of proteins