ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

NCLIV_045650 (GRA2)

28 kDa antigen, putative, a gene from Neospora caninum

Compiled localisation
cyst wall during bradyzoite
anterior cytoplasm and posterior cytoplasm during tachyzoite

Who localised this protein by microscopy?

Vonlaufen, N., Guetg, N., Naguleswaran, A., Muller, N., Bjorkman, C., Schares, G., von Blumroeder, D., Ellis, J., Hemphill, A.
In vitro induction of Neospora caninum bradyzoites in vero cells reveals differential antigen expression, localization, and host-cell recognition of tachyzoites and bradyzoites. (2004 Jan, Infect Immun) PubMed / full text
   more detail about this publication

cyst wall during bradyzoite, anterior cytoplasm and posterior cytoplasm during tachyzoite
  • "Granular-type immunolabeling was found predominantly within the parasite cytoplasm at the anterior and posterior ends of the tachyzoites. In contrast, bradyzoites exhibited staining for NcGRA1 (see Fig. 4D to F) and NcGRA7 (see Fig. 4J to L) that had largely shifted toward the periphery of the vacuole. For NcGRA2 (see Fig. 4G to I), peripheral labeling was not as pronounced but was clearly evident as well."
  • Microscopy type: light, EM
  • Microscopy method: polylonal antibody
  • Strain:
  • Gene model mapping comments: blast from AF196293, annotation matches
  • Localisation record: cyst wall during bradyzoite, posterior cytoplasm and anterior cytoplasm during tachyzoite
  • Comment: EM not shown in paper
  • Other genes localised in this publication: dense granule protein 1 / major antigenp24, putative, dense granule protein 7, putative

Orthology

OrthoMCL

Part of OrthoMCL group OG4_115531

Localised Genes in this Group
Other Apicomplexan Genes in this Group
    There are no other apicomplexan genes.
Non-Apicomplexan Genes in this OrthoMCL Group with Gene Ontology 'Inferred from Direct Assay (IDA)' Cellular Component Terms
    There are no non-apicomplexan genes that have manually annotated evidence codes.

Amino Acid Sequence

>NCLIV_045650 28 kDa antigen, putative
MFTGKRWILVVAVGALVGASVKAADFSGRGTVNGQPVGSGYSGYPRGDDVRESMAAPEDLPGERQPETPTAEAVKQAAAK
AYRLLKQFTAKVGQETENAYYHVKKATMKGFDVAKDQSYKGYLAVRKATAKGLQSAGKSLELKESAPTGTTTAAPTEKVP
PSGPRSGEVQRTRKEQNDVQQTAEMLAEEILEAGLKKDDGEGRGTPEAEVN

Proteomics

Found in:

Not found in:

Gene-Specific Links for NCLIV_045650

General Sub-Cellular Localisation Links

  • TargetP, prediction of signal peptides, as well as chloroplast and mitochondrial transit peptides
  • OrthoMCL, automatic clustering of orthologous groups of proteins