ApiLoc - A database of published protein sub-cellular localisation in Apicomplexa

version 3 (curated until May 28, 2011)

No assigned gene identifier (MIC3, -P38, MIC4, -p43, BAG1, SAG1, p29, SRS2, p35)

A gene from Neospora caninum

Compiled localisation
dense granule and microneme during tachyzoite
cell surface during tachyzoite
rhoptry during tachyzoite
cytoplasm during bradyzoite
during not bradyzoite and not tachyzoite

Who localised this protein by microscopy?

Keller, N., Riesen, M., Naguleswaran, A., Vonlaufen, N., Stettler, R., Leepin, A., Wastling, J. M., Hemphill, A.
Identification and characterization of a Neospora caninum microneme-associated protein (NcMIC4) that exhibits unique lactose-binding properties. (2004 Aug, Infect Immun) PubMed / full text
   more detail about this publication

microneme during tachyzoite
  • "Immunofluorescence and immunogold electron microscopy with affinity-purified antibodies showed that the protein was associated with the tachyzoite micronemes"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from BK005222, no matching gene model found
  • Localisation record: microneme during tachyzoite
  • Other genes localised in this publication: -
  • "Immunofluorescence and immunogold electron microscopy with affinity-purified antibodies showed that the protein was associated with the tachyzoite micronemes"
  • Microscopy type: Light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from DAA05464, no matching gene model found
  • Localisation record: microneme
  • Other genes localised in this publication: -

Sonda, S., Fuchs, N., Gottstein, B., Hemphill, A.
Molecular characterization of a novel microneme antigen in Neospora caninum. (2000 Apr 30, Mol Biochem Parasitol) PubMed / full text
   more detail about this publication

dense granule and microneme during tachyzoite
  • "By immunofluorescence, these antibodies stained predominantly the apical portion of both N. caninum and T. gondii tachyzoites, but the protein was absent from the parasite surface. Immunogold localization in LR-White embedded N. caninum tachyzoites demonstrated staining of predominantly the apically located micronemes, as well as of dense granules located at the posterior end of the tachyzoites"
  • Microscopy type: light, EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from AF198615, no matching gene model found
  • Localisation record: microneme during tachyzoite, dense granule during tachyzoite
  • Other genes localised in this publication: -

Weiss, L. M., Ma, Y. F., Halonen, S., McAllister, M. M., Zhang, Y. W.
The in vitro development of Neospora caninum bradyzoites. (1999 Oct, Int J Parasitol) PubMed / full text
   more detail about this publication

cytoplasm during bradyzoite, during not tachyzoite
  • "This reaction was localised primarily to the cytoplasm of the parasites"
  • Microscopy type: light, EM
  • Microscopy method: polyclonal antibody to homologue, monoclonal antibody to homologue
  • Strain:
  • Gene model mapping comments: blast from U23944, doesn't match perfectly to genome or gene models – too fast evolving?
  • Localisation record: cytoplasm during bradyzoite, not during tachyzoite
  • Other genes localised in this publication: -

Howe, D. K., Crawford, A. C., Lindsay, D., Sibley, L. D.
The p29 and p35 immunodominant antigens of Neospora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii. (1998 Nov, Infect Immun) PubMed / full text
   more detail about this publication

cell surface during tachyzoite, during not bradyzoite
  • "Localization studies and surface labeling with biotin demonstrated that Ncp29 and Ncp35 are membrane associated and displayed on the surface of the parasite"
  • Microscopy type: EM
  • Microscopy method: monoclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from AAD25091, no matching gene model
  • Localisation record: parasite surface during tachyzoite
  • Other genes localised in this publication: -
  • "Localization studies and surface labeling with biotin demonstrated that Ncp29 and Ncp35 are membrane associated and displayed on the surface of the parasite"
  • Microscopy type: EM
  • Microscopy method: monoclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from AAD04844, no matching gene model
  • Localisation record: parasite surface during tachyzoite, not during bradyzoite
  • Other genes localised in this publication: -

Hemphill, A.
Subcellular localization and functional characterization of Nc-p43, a major Neospora caninum tachyzoite surface protein. (1996 Oct, Infect Immun) PubMed / full text
   more detail about this publication

cell surface and dense granule and rhoptry during tachyzoite
  • "Postembedding immunogold labeling with anti-Nc-p43 antibodies demonstrated that Nc-p43 is localized not only on the parasite cell surface but also within dense granules and rhoptries."
  • Microscopy type: EM
  • Microscopy method: polyclonal antibody directly to protein
  • Strain:
  • Gene model mapping comments: blast from U93870, no matching gene model
  • Localisation record: surface and dense granule and rhoptry during tachyzoite
  • Other genes localised in this publication: -

Orthology

OrthoMCL

No entry found in OrthoMCL.

Amino Acid Sequence

amino acid sequence not recorded

Proteomics

Found in:

Not found in:

Gene-Specific Links for A common gene for all genes not assigned to a gene model

General Sub-Cellular Localisation Links

  • TargetP, prediction of signal peptides, as well as chloroplast and mitochondrial transit peptides
  • OrthoMCL, automatic clustering of orthologous groups of proteins